Polimorfismo del gen TLR4 asociado a caracteres de producción de leche en vacas Holstein manejadas bajo estrés por calor
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Publicado:
dic 4, 2024
Palabras clave:
ADN, estrés por calor, ITH, lactantes, SNP
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Resumen
Los establos de ganado bovino para leche en el estado de Sonora se caracterizan por una producción anual contínua, la cual se ve seriamente afectada por las condiciones adversas de temperatura y humedad ambiental que prevalecen gran parte de año, ya que superan el umbral de confort térmico de la vaca conduciendo al estrés por calor. No obstante el reto que representan estas condiciones para los sistemas de producción de leche, es posible observar vacas con habilidad para mantener parámetros productivos eficientes, lo cual se atribuye a la existencia de genes que regulan producción de leche y termotolerancia. Por lo tanto, el objetivo de este estudio fue evaluar un polimorfismo del gen TLR4 como marcador genético para caracteres asociados a la producción de leche en vacas Holstein manejadas bajo estrés por calor. El estudio incluyó 264 vacas lactantes de dos establos ubicados en el Valle del Yaqui. Se registró la producción de leche total durante la lactancia y se ajustó a 305 días, calculando los valores promedio diarios de ambas mediciones. Se obtuvieron datos climatológicos para calcular el indicador de temperatura-humedad (ITH), el cual indicó la presencia de estrés por calor de abril a octubre. Muestras de sangre fueron obtenidas de cada vaca y procesadas para la extracción de ADN, el cual fue utilizado para el genotipado de un polimorfismo de nucleótido simple (SNP), del gen del receptor tipo toll-4 (TLR4) a través del ensayo TaqMan y la prueba de qPCR. Un modelo estadístico de efectos mixtos validó el efecto predictor (P < 0.05), del SNP rs8193046 sobre 4 rasgos asociados a la producción de leche y confirmó el comportamiento aditivo del gen TLR4. El genotipo favorable resultó ser el genotipo TT, donde cada alelo T mostró una contribución (P < 0.01), en la mejora de los 4 parámetros evaluados. En conclusión, se propone al SNP rs8193046 del gen TLR4 como marcador genético candidato asociado a la producción de leche en vacas Holstein manejadas bajo condiciones ambientales de estrés por calor.
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Hurtado-Martínez, R. L., Pineda-Montes, A., Luna-Ramirez, P., Reyna-Granados, J. R., Leyva-Corona, J. C., Zamorano-Algandar, R., Nieblas- López, M. I., & Luna-Nevárez, P. (2024). Polimorfismo del gen TLR4 asociado a caracteres de producción de leche en vacas Holstein manejadas bajo estrés por calor . Revista Latinoamericana De Recursos Naturales, 20(3), 138-144. Recuperado a partir de https://revista.itson.edu.mx/index.php/rlrn/article/view/351
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Collier, R.J., Collier, J.L., Rhoads, R.P., Baumgard, L.H. 2008. Invited Review: Genes Involved in the Bovine Heat Stress Response. J. Dairy Sci. 91: 445-454, https://doi.org/10.3168/jds.2007-0540
Contreras-Méndez, L.A., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Luna-Nevárez, G., López-Castro, P.A., Rivera-Acuña, F., Luna-Nevárez, P. 2024. The AntiMüllerian hormone as endocrine and molecular marker associated with reproductive performance in Holstein dairy cows exposed to heat stress. Animals (Basel), 14(2):213, https://doi.org/10.3390/ani14020213
Correa, A., Avendaño, L., López, M., Macías, U. 2022. Estrés por calor en ganado lechero con énfasis en la producción de leche y los hábitos de consumo de alimento y agua. Revisión. Revista mexicana de ciencias pecuarias, 13(2), 488-509, https://doi.org/10.22319/rmcp.v13i2.5832
Do Amaral, B.C., Connor, E.E., Tao, S., Hayen, J., Bubolz, J., Dahl, G.E. 2010. Heat stress abatement during the dry period influences prolactin signaling in lymphocytes. Domestic Animal Endocrinology, 38, 38–45, https://doi.org/10.1016/j.domaniend.2009.07.005
Duan, T., Du, Y., Xing, C., Wang, H.Y., Wang, R.F. 2022. TollLike Receptor Signaling and Its Role in Cell-Mediated Immunity. Frontiers in Immunology, 13, https://doi.org/10.3389/fimmu.2022.812774
Gupta, S. Sharma, A. Joy, A. Dunshea, F.R., Chauhan, S.S. 2022. The impact of heat stress on immune status of dairy cattle and strategies to ameliorate the negative effects. Animals, 13, 107, https://doi.org/10.3390/ani13010107
Henry, B., Eckard, R., Beauchemin, K. 2018. Adaptation of ruminant livestock production systems to climate changes. Animal, 12, s445–s456. https://doi.org/10.1017/S1751731118001301
Hernández-Cordero, A.I., Sánchez-Castro, M.A., ZamoranoAlgandar, R., Luna-Nevárez, P., Rincón, G., Medrano, J.F., Speidel, S.E., Enns, R.M., Thomas, M.G. 2018. Genotypes within the Prolactin and Growth Hormone Insulin-Like Growth Factor-I pathways associated with milk production in heat stressed Holstein cattle. Gen. Mol. Res., 16(4), 1-10, http://dx.doi.org/10.4238/gmr16039821
Hong, E.P., Park, J.W. 2012. Sample size and statistical power calculation in genetic association studies. Genom. Inform. 10, 117–122, https://doi.org/10.5808/GI.2012.10.2.117
Jiang, J., Ma, L., Prakapenka, D., VanRaden, P.M., Cole, J.B., Da, Y. 2019. A large-scale genome-wide association study in U.S. Holstein cattle. Front. Genet., 14, 412. https://doi.org/10.3389/fgene.2019.00412
Leyva-Corona, J.C., Reyna-Granados, J.R., Zamorano-Algandar, R., Sánchez-Castro, M.A., Thomas, M.G., Enns, R.M., Speidel, S.E., Medrano, J.F., Rincon, G., Luna-Nevárez, P. 2018. Polymorphisms within the prolactin and growth hormone/insulin-like growth factor-1 functional pathways associated with fertility traits in Holstein cows raised in a hot-humid climate. 50(8), 1913–1920, https://doi.org/10.1007/s11250-018-1645-0
Luna-Nevarez, P., Rincon, G., Medrano, J.F., Riley, D.G., Chace, C. Jr., Coleman, S.W., DeAtley, K.L., Islas-Trejo, A., Silver, G.A., Thomas, M.G. 2012. Identificación de un polimorfismo del gen PAPP-A2 asociado a la fertilidad en vaquillas Romosinuano criadas en subtropico. Revista Mexicana de Ciencias Pecuarias, 3(2), 185-200.
Liu, J., Li, L., Chen, X., Lu, Y., Wang, D. 2019. Effects of heat stress on body temperature, milk production, and reproduction in dairy cows: a novel idea for monitoring and evaluation of heat stress — A review. Asian-Australasian Journal of Animal Sciences, 32(9), 1332–1339, https://doi.org/10.5713/ajas.18.0743
Liu, Z., Ezernieks, V., Wang, J., Arachchillage, N.W., Garner, J.B., Wales, W.J., Cocks, B.G., Rochfort, S. 2017. Heat stress in dairy cattle alters lipid composition of milk. Sci. Rep., 7, 961, https://doi.org/10.1038/s41598-017-01120-9
Macciotta, N.P.P., Biffani, S., Bernabucci, U., Lacetera, N., Vitali, A., Ajmone-Marsan, P. Nardone, A. 2017. Derivation and genome-wide association study of a principal component-based measure of heat tolerance in dairy cattle. J. Dairy Sci., 100, 4683–4697, https://doi.org/10.3168/jds.2016-12249
Mader, T.L., Davis, M.S., Brown-Brandl, T. 2006. Environmental factors influencing heat stress in feedlot cattle1,2. Journal of Animal Science, 84(3), 712–719, https://doi.org/10.2527/2006.843712x
Mariasegaram, M., Chase, C.C., Chaparro, J.X., Olson, T.A., Brenneman, R.A., Niedz, R.P. 2007. The slick hair coat locus maps to chromosome 20 in Senepol-derived Cattle. Animal Genetics, 38:54-59, https://doi.org/10.1111/j.1365-
2052.2007.01560.x
Meuwissen, T., Hayes, B., Goddard, M. 2016. Genomic selection: A paradigm shifts in animal breeding, Animal Frontiers, 6, 6-14, https://doi.org/10.2527/af.2016-0002
Misztal, I., Hayes, B.J., VanRaden, P.M. 2015. Implications of genome-wide association studies in dairy cattle breeding theory. Journal of Dairy Science, 98(5), 3361-3377, https://doi.org/10.13140/RG.2.1.4504.1760
Proaño-Morales, A.B., Luna-Nevárez, G., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., ZamoranoAlgandar, R., Sánchez-Castro, M.A., Leyva-Corona, J.C., Luna-Nevárez, P. 2021. Marcadores genéticos asociados a un indicador de tolerancia al estrés por calor en vacas Holstein lactantes del sur de Sonora México. Revista Latinoamericana de Recursos Naturales. 17(2): 55-62.
Sharma, A., Lee, J.D., Dang, C.G., Sudrajad, P., Kim, H.C., Yeon, S.H., Kang, H.S., Lee, S.H. 2015. Stories and Challenges of Genome Wide Association Studies in Livestock — A Review. 28(10), 1371–1379, https://doi.org/10.5713/ajas.14.0715
Sigdel, A., Abdollahi-Arpanahi, R., Aguilar, I., Peñagaricano, F. 2019. Whole genome mapping reveals novel genes and pathways involved in milk production under heat stress in US Holstein cows. Front. Genet., 4, 928, https://doi.org/10.3389/fgene.2019.00928
Silva, M.V.B., dos Santos, D.J.A., Boison, S.A., Utsunomiya, A.T.H., Carmo, A.S., Sonstegard, T.S., Cole, J.B., Van Tassell, C.P. 2014. The development of genomics applied to dairy breeding, Livestock Science, 166, 66-75., https://doi.org/10.1016/j.livsci.2014.05.017
Wang, M., Song, H.L., Zhu, X., Xing, S., Zhang, M., Zhang, H., Wang, X., Yang, Z., Ding, X., Karrow, N.A., König, S., Mao, Y. 2018. Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows. 85(4), 407–411, https://doi.org/10.1017/s0022029918000535
Zamorano‐Algandar, R., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Sánchez-Castro, M.A., Luna‐Nevárez, G., Leyva-Corona, J.C., Luna‐Nevárez, P. 2023. Genetic Markers Associated with Milk Production and Thermotolerance in Holstein Dairy Cows Managed in a Heat-Stressed Environment. Biology, 12(5), 679–679, https://doi.org/10.3390/biology12050679
Chen, H., Liu, C., Xiang, M., Yu, J., Xia, Y., Hu, X., Wang, D., Tao, B., Zhang, Y., Cheng, L. 2022. Contribution of the mutation rs8193069 in TLR4 to mastitis resistance and performance in Holstein cows in southern China. Veterinary Medicine and Science, 8(1): 357–366. https://doi.org/10.1002/vms3.671
Collier, R.J., Collier, J.L., Rhoads, R.P., Baumgard, L.H. 2008. Invited Review: Genes Involved in the Bovine Heat Stress Response. J. Dairy Sci. 91: 445-454, https://doi.org/10.3168/jds.2007-0540
Contreras-Méndez, L.A., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Luna-Nevárez, G., López-Castro, P.A., Rivera-Acuña, F., Luna-Nevárez, P. 2024. The AntiMüllerian hormone as endocrine and molecular marker associated with reproductive performance in Holstein dairy cows exposed to heat stress. Animals (Basel), 14(2):213, https://doi.org/10.3390/ani14020213
Correa, A., Avendaño, L., López, M., Macías, U. 2022. Estrés por calor en ganado lechero con énfasis en la producción de leche y los hábitos de consumo de alimento y agua. Revisión. Revista mexicana de ciencias pecuarias, 13(2), 488-509, https://doi.org/10.22319/rmcp.v13i2.5832
Do Amaral, B.C., Connor, E.E., Tao, S., Hayen, J., Bubolz, J., Dahl, G.E. 2010. Heat stress abatement during the dry period influences prolactin signaling in lymphocytes. Domestic Animal Endocrinology, 38, 38–45, https://doi.org/10.1016/j.domaniend.2009.07.005
Duan, T., Du, Y., Xing, C., Wang, H.Y., Wang, R.F. 2022. TollLike Receptor Signaling and Its Role in Cell-Mediated Immunity. Frontiers in Immunology, 13, https://doi.org/10.3389/fimmu.2022.812774
Gupta, S. Sharma, A. Joy, A. Dunshea, F.R., Chauhan, S.S. 2022. The impact of heat stress on immune status of dairy cattle and strategies to ameliorate the negative effects. Animals, 13, 107, https://doi.org/10.3390/ani13010107
Henry, B., Eckard, R., Beauchemin, K. 2018. Adaptation of ruminant livestock production systems to climate changes. Animal, 12, s445–s456. https://doi.org/10.1017/S1751731118001301
Hernández-Cordero, A.I., Sánchez-Castro, M.A., ZamoranoAlgandar, R., Luna-Nevárez, P., Rincón, G., Medrano, J.F., Speidel, S.E., Enns, R.M., Thomas, M.G. 2018. Genotypes within the Prolactin and Growth Hormone Insulin-Like Growth Factor-I pathways associated with milk production in heat stressed Holstein cattle. Gen. Mol. Res., 16(4), 1-10, http://dx.doi.org/10.4238/gmr16039821
Hong, E.P., Park, J.W. 2012. Sample size and statistical power calculation in genetic association studies. Genom. Inform. 10, 117–122, https://doi.org/10.5808/GI.2012.10.2.117
Jiang, J., Ma, L., Prakapenka, D., VanRaden, P.M., Cole, J.B., Da, Y. 2019. A large-scale genome-wide association study in U.S. Holstein cattle. Front. Genet., 14, 412. https://doi.org/10.3389/fgene.2019.00412
Leyva-Corona, J.C., Reyna-Granados, J.R., Zamorano-Algandar, R., Sánchez-Castro, M.A., Thomas, M.G., Enns, R.M., Speidel, S.E., Medrano, J.F., Rincon, G., Luna-Nevárez, P. 2018. Polymorphisms within the prolactin and growth hormone/insulin-like growth factor-1 functional pathways associated with fertility traits in Holstein cows raised in a hot-humid climate. 50(8), 1913–1920, https://doi.org/10.1007/s11250-018-1645-0
Luna-Nevarez, P., Rincon, G., Medrano, J.F., Riley, D.G., Chace, C. Jr., Coleman, S.W., DeAtley, K.L., Islas-Trejo, A., Silver, G.A., Thomas, M.G. 2012. Identificación de un polimorfismo del gen PAPP-A2 asociado a la fertilidad en vaquillas Romosinuano criadas en subtropico. Revista Mexicana de Ciencias Pecuarias, 3(2), 185-200.
Liu, J., Li, L., Chen, X., Lu, Y., Wang, D. 2019. Effects of heat stress on body temperature, milk production, and reproduction in dairy cows: a novel idea for monitoring and evaluation of heat stress — A review. Asian-Australasian Journal of Animal Sciences, 32(9), 1332–1339, https://doi.org/10.5713/ajas.18.0743
Liu, Z., Ezernieks, V., Wang, J., Arachchillage, N.W., Garner, J.B., Wales, W.J., Cocks, B.G., Rochfort, S. 2017. Heat stress in dairy cattle alters lipid composition of milk. Sci. Rep., 7, 961, https://doi.org/10.1038/s41598-017-01120-9
Macciotta, N.P.P., Biffani, S., Bernabucci, U., Lacetera, N., Vitali, A., Ajmone-Marsan, P. Nardone, A. 2017. Derivation and genome-wide association study of a principal component-based measure of heat tolerance in dairy cattle. J. Dairy Sci., 100, 4683–4697, https://doi.org/10.3168/jds.2016-12249
Mader, T.L., Davis, M.S., Brown-Brandl, T. 2006. Environmental factors influencing heat stress in feedlot cattle1,2. Journal of Animal Science, 84(3), 712–719, https://doi.org/10.2527/2006.843712x
Mariasegaram, M., Chase, C.C., Chaparro, J.X., Olson, T.A., Brenneman, R.A., Niedz, R.P. 2007. The slick hair coat locus maps to chromosome 20 in Senepol-derived Cattle. Animal Genetics, 38:54-59, https://doi.org/10.1111/j.1365-
2052.2007.01560.x
Meuwissen, T., Hayes, B., Goddard, M. 2016. Genomic selection: A paradigm shifts in animal breeding, Animal Frontiers, 6, 6-14, https://doi.org/10.2527/af.2016-0002
Misztal, I., Hayes, B.J., VanRaden, P.M. 2015. Implications of genome-wide association studies in dairy cattle breeding theory. Journal of Dairy Science, 98(5), 3361-3377, https://doi.org/10.13140/RG.2.1.4504.1760
Proaño-Morales, A.B., Luna-Nevárez, G., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., ZamoranoAlgandar, R., Sánchez-Castro, M.A., Leyva-Corona, J.C., Luna-Nevárez, P. 2021. Marcadores genéticos asociados a un indicador de tolerancia al estrés por calor en vacas Holstein lactantes del sur de Sonora México. Revista Latinoamericana de Recursos Naturales. 17(2): 55-62.
Sharma, A., Lee, J.D., Dang, C.G., Sudrajad, P., Kim, H.C., Yeon, S.H., Kang, H.S., Lee, S.H. 2015. Stories and Challenges of Genome Wide Association Studies in Livestock — A Review. 28(10), 1371–1379, https://doi.org/10.5713/ajas.14.0715
Sigdel, A., Abdollahi-Arpanahi, R., Aguilar, I., Peñagaricano, F. 2019. Whole genome mapping reveals novel genes and pathways involved in milk production under heat stress in US Holstein cows. Front. Genet., 4, 928, https://doi.org/10.3389/fgene.2019.00928
Silva, M.V.B., dos Santos, D.J.A., Boison, S.A., Utsunomiya, A.T.H., Carmo, A.S., Sonstegard, T.S., Cole, J.B., Van Tassell, C.P. 2014. The development of genomics applied to dairy breeding, Livestock Science, 166, 66-75., https://doi.org/10.1016/j.livsci.2014.05.017
Wang, M., Song, H.L., Zhu, X., Xing, S., Zhang, M., Zhang, H., Wang, X., Yang, Z., Ding, X., Karrow, N.A., König, S., Mao, Y. 2018. Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows. 85(4), 407–411, https://doi.org/10.1017/s0022029918000535
Zamorano‐Algandar, R., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Sánchez-Castro, M.A., Luna‐Nevárez, G., Leyva-Corona, J.C., Luna‐Nevárez, P. 2023. Genetic Markers Associated with Milk Production and Thermotolerance in Holstein Dairy Cows Managed in a Heat-Stressed Environment. Biology, 12(5), 679–679, https://doi.org/10.3390/biology12050679