Polymorphism from the gene TLR4 associated with milk production traits in Holstein cows managed under heat stress
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Published:
Dec 4, 2024
Keywords:
DNA, heat stress, lactating, SNP, THI
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Abstract
Dairy cattle farms in Sonora are characterized by continuous annual production, which is seriously affected by adverse environmental temperature and humidity conditions that prevail most of the year, as they exceed the cow's thermal comfort threshold, leading to heat stress. Despite the challenge these conditions represent for milk production systems, it is possible to observe cows with the ability to maintain efficient production parameters, which is attributed to a genetic basis. Therefore, the objective of this study was to evaluate a polymorphism of the TLR4 gene as a genetic marker for traits associated with milk production in Holstein cows managed under heat stress. The study included 264 lactating cows from two farms located in the Yaqui Valley. Total milk production during lactation was recorded and adjusted to 305 days. Climatological data were obtained to calculate the THI, which indicated the presence of heat stress from April to October. Blood samples were obtained from each cow and processed for DNA extraction, which was used for genotyping of a single nucleotide polymorphism (SNP) of the toll-like receptor 4 (TLR4) gene by TaqMan assay and qPCR test. A mixed effects statistical model validated the predictive effect (P < 0.05) of the SNP rs8193046 on four traits associated with milk production and confirmed the additive behavior of the TLR4 gene. The favorable genotype was the TT genotype, where each T allele showed a contribution (P < 0.01) in the improvement of the four parameters evaluated. In conclusion, the SNP rs8193046 of the TLR4 gene is proposed as a genetic marker associated with milk production in Holstein cows managed under heat stress.
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Hurtado-Martínez, R. L., Pineda-Montes, A., Luna-Ramirez, P., Reyna-Granados, J. R., Leyva-Corona, J. C., Zamorano-Algandar, R., Nieblas- López, M. I., & Luna-Nevárez, P. (2024). Polymorphism from the gene TLR4 associated with milk production traits in Holstein cows managed under heat stress. Revista Latinoamericana De Recursos Naturales, 20(3), 138-144. Retrieved from https://revista.itson.edu.mx/index.php/rlrn/article/view/351
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References
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Chen, H., Liu, C., Xiang, M., Yu, J., Xia, Y., Hu, X., Wang, D., Tao, B., Zhang, Y., Cheng, L. 2022. Contribution of the mutation rs8193069 in TLR4 to mastitis resistance and performance in Holstein cows in southern China. Veterinary Medicine and Science, 8(1): 357–366. https://doi.org/10.1002/vms3.671
Collier, R.J., Collier, J.L., Rhoads, R.P., Baumgard, L.H. 2008. Invited Review: Genes Involved in the Bovine Heat Stress Response. J. Dairy Sci. 91: 445-454, https://doi.org/10.3168/jds.2007-0540
Contreras-Méndez, L.A., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Luna-Nevárez, G., López-Castro, P.A., Rivera-Acuña, F., Luna-Nevárez, P. 2024. The AntiMüllerian hormone as endocrine and molecular marker associated with reproductive performance in Holstein dairy cows exposed to heat stress. Animals (Basel), 14(2):213, https://doi.org/10.3390/ani14020213
Correa, A., Avendaño, L., López, M., Macías, U. 2022. Estrés por calor en ganado lechero con énfasis en la producción de leche y los hábitos de consumo de alimento y agua. Revisión. Revista mexicana de ciencias pecuarias, 13(2), 488-509, https://doi.org/10.22319/rmcp.v13i2.5832
Do Amaral, B.C., Connor, E.E., Tao, S., Hayen, J., Bubolz, J., Dahl, G.E. 2010. Heat stress abatement during the dry period influences prolactin signaling in lymphocytes. Domestic Animal Endocrinology, 38, 38–45, https://doi.org/10.1016/j.domaniend.2009.07.005
Duan, T., Du, Y., Xing, C., Wang, H.Y., Wang, R.F. 2022. TollLike Receptor Signaling and Its Role in Cell-Mediated Immunity. Frontiers in Immunology, 13, https://doi.org/10.3389/fimmu.2022.812774
Gupta, S. Sharma, A. Joy, A. Dunshea, F.R., Chauhan, S.S. 2022. The impact of heat stress on immune status of dairy cattle and strategies to ameliorate the negative effects. Animals, 13, 107, https://doi.org/10.3390/ani13010107
Henry, B., Eckard, R., Beauchemin, K. 2018. Adaptation of ruminant livestock production systems to climate changes. Animal, 12, s445–s456. https://doi.org/10.1017/S1751731118001301
Hernández-Cordero, A.I., Sánchez-Castro, M.A., ZamoranoAlgandar, R., Luna-Nevárez, P., Rincón, G., Medrano, J.F., Speidel, S.E., Enns, R.M., Thomas, M.G. 2018. Genotypes within the Prolactin and Growth Hormone Insulin-Like Growth Factor-I pathways associated with milk production in heat stressed Holstein cattle. Gen. Mol. Res., 16(4), 1-10, http://dx.doi.org/10.4238/gmr16039821
Hong, E.P., Park, J.W. 2012. Sample size and statistical power calculation in genetic association studies. Genom. Inform. 10, 117–122, https://doi.org/10.5808/GI.2012.10.2.117
Jiang, J., Ma, L., Prakapenka, D., VanRaden, P.M., Cole, J.B., Da, Y. 2019. A large-scale genome-wide association study in U.S. Holstein cattle. Front. Genet., 14, 412. https://doi.org/10.3389/fgene.2019.00412
Leyva-Corona, J.C., Reyna-Granados, J.R., Zamorano-Algandar, R., Sánchez-Castro, M.A., Thomas, M.G., Enns, R.M., Speidel, S.E., Medrano, J.F., Rincon, G., Luna-Nevárez, P. 2018. Polymorphisms within the prolactin and growth hormone/insulin-like growth factor-1 functional pathways associated with fertility traits in Holstein cows raised in a hot-humid climate. 50(8), 1913–1920, https://doi.org/10.1007/s11250-018-1645-0
Luna-Nevarez, P., Rincon, G., Medrano, J.F., Riley, D.G., Chace, C. Jr., Coleman, S.W., DeAtley, K.L., Islas-Trejo, A., Silver, G.A., Thomas, M.G. 2012. Identificación de un polimorfismo del gen PAPP-A2 asociado a la fertilidad en vaquillas Romosinuano criadas en subtropico. Revista Mexicana de Ciencias Pecuarias, 3(2), 185-200.
Liu, J., Li, L., Chen, X., Lu, Y., Wang, D. 2019. Effects of heat stress on body temperature, milk production, and reproduction in dairy cows: a novel idea for monitoring and evaluation of heat stress — A review. Asian-Australasian Journal of Animal Sciences, 32(9), 1332–1339, https://doi.org/10.5713/ajas.18.0743
Liu, Z., Ezernieks, V., Wang, J., Arachchillage, N.W., Garner, J.B., Wales, W.J., Cocks, B.G., Rochfort, S. 2017. Heat stress in dairy cattle alters lipid composition of milk. Sci. Rep., 7, 961, https://doi.org/10.1038/s41598-017-01120-9
Macciotta, N.P.P., Biffani, S., Bernabucci, U., Lacetera, N., Vitali, A., Ajmone-Marsan, P. Nardone, A. 2017. Derivation and genome-wide association study of a principal component-based measure of heat tolerance in dairy cattle. J. Dairy Sci., 100, 4683–4697, https://doi.org/10.3168/jds.2016-12249
Mader, T.L., Davis, M.S., Brown-Brandl, T. 2006. Environmental factors influencing heat stress in feedlot cattle1,2. Journal of Animal Science, 84(3), 712–719, https://doi.org/10.2527/2006.843712x
Mariasegaram, M., Chase, C.C., Chaparro, J.X., Olson, T.A., Brenneman, R.A., Niedz, R.P. 2007. The slick hair coat locus maps to chromosome 20 in Senepol-derived Cattle. Animal Genetics, 38:54-59, https://doi.org/10.1111/j.1365-
2052.2007.01560.x
Meuwissen, T., Hayes, B., Goddard, M. 2016. Genomic selection: A paradigm shifts in animal breeding, Animal Frontiers, 6, 6-14, https://doi.org/10.2527/af.2016-0002
Misztal, I., Hayes, B.J., VanRaden, P.M. 2015. Implications of genome-wide association studies in dairy cattle breeding theory. Journal of Dairy Science, 98(5), 3361-3377, https://doi.org/10.13140/RG.2.1.4504.1760
Proaño-Morales, A.B., Luna-Nevárez, G., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., ZamoranoAlgandar, R., Sánchez-Castro, M.A., Leyva-Corona, J.C., Luna-Nevárez, P. 2021. Marcadores genéticos asociados a un indicador de tolerancia al estrés por calor en vacas Holstein lactantes del sur de Sonora México. Revista Latinoamericana de Recursos Naturales. 17(2): 55-62.
Sharma, A., Lee, J.D., Dang, C.G., Sudrajad, P., Kim, H.C., Yeon, S.H., Kang, H.S., Lee, S.H. 2015. Stories and Challenges of Genome Wide Association Studies in Livestock — A Review. 28(10), 1371–1379, https://doi.org/10.5713/ajas.14.0715
Sigdel, A., Abdollahi-Arpanahi, R., Aguilar, I., Peñagaricano, F. 2019. Whole genome mapping reveals novel genes and pathways involved in milk production under heat stress in US Holstein cows. Front. Genet., 4, 928, https://doi.org/10.3389/fgene.2019.00928
Silva, M.V.B., dos Santos, D.J.A., Boison, S.A., Utsunomiya, A.T.H., Carmo, A.S., Sonstegard, T.S., Cole, J.B., Van Tassell, C.P. 2014. The development of genomics applied to dairy breeding, Livestock Science, 166, 66-75., https://doi.org/10.1016/j.livsci.2014.05.017
Wang, M., Song, H.L., Zhu, X., Xing, S., Zhang, M., Zhang, H., Wang, X., Yang, Z., Ding, X., Karrow, N.A., König, S., Mao, Y. 2018. Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows. 85(4), 407–411, https://doi.org/10.1017/s0022029918000535
Zamorano‐Algandar, R., Medrano, J.F., Thomas, M.G., Enns, R.M., Speidel, S.E., Sánchez-Castro, M.A., Luna‐Nevárez, G., Leyva-Corona, J.C., Luna‐Nevárez, P. 2023. Genetic Markers Associated with Milk Production and Thermotolerance in Holstein Dairy Cows Managed in a Heat-Stressed Environment. Biology, 12(5), 679–679, https://doi.org/10.3390/biology12050679