Construction of a high performance liquid chromatography system and column for metal analysis
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Abstract
It was constructed an equipment for High Performance Liquid Chromatography with a syringe pump, an injector port for 1 μl sample size, a column prepared in situ for cationic exchange with SO3- as active group, for the separation of metals as Al, Zn, Cd, Mg and Ca, that were complexed with HQS 0.2 mM at a flowrate of 3 μl/min, before its separation into the column and detected by a fluorescence detector, to obtain a detection limit of 0.2, 0.4, 0.6, 5 and 8 pg of Al, Zn, Cd, Mg nd Ca, respectively.
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Villanueva-Fierro, I. (2009). Construction of a high performance liquid chromatography system and column for metal analysis. Revista Latinoamericana De Recursos Naturales, 5(2), 138-140. Retrieved from https://revista.itson.edu.mx/index.php/rlrn/article/view/163
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
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Dasgupta Purnendu K., In-YongEom, Kavin J. Morris, Jianzhong Li. 2003. Light emitting diode-based detectors Absorbance, fluorescence and spectroelectrochemical measurements in a planar flow-through cell. Analytical Chimica Acta. 500, 337-364.
García-Reyes Juan F., Pilar Ortega-Barrales, Antonio Molina- Díaz. 2006. Sensing of trace amounts of cadmium in drinking water using a single fluorescence-based optosensor. Microchemical Journal. 82, 94-99.
Soroka Krystyna, Rathnapala S. Vithanage, Denise A. Phillips, Brian Walker, and Purnendu K. Dasgupta, 1987. Fluorescence properties of metal compexes of 8- hydroxyquinoline-s-sulfonic acid and chromatographic applications. Analytical Chemistry. 59, 629-636.
Svec Frantisek, and Jean M. J. Fréchet. 1992. Continuous rods of macroporous polymer as high-peformance liquid chromatography separation media. Anal. Chem. 64, 820- 822.
Williams Tim and Neil W. Barnett. 1992. 8-Quinolinol-5- sulfonic acid as a non-selective post-column reagent for fluorimetric detection of trace metals in ion chromatography. Analytica Chimica Acta. 264, 297-301.