In vitro clonal propagation, conservation and international transference of germplasm of cassava (Manihot esculenta Crantz) native of Peru
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Abstract
Accessions of cassava (Manihot esculenta Crantz) collected in Peru and preserved in the Germplasm Bank of Universidad Nacional Pedro Ruiz Gallo (UNPRG), Lambayeque, Peru, were evaluated in both meristem and germplasm conservation culture medium. These culture media, conventionally called 4E and 8S, respectively, were formulated at the International Center for Tropical Agriculture (CIAT) Cali, Colombia and are widely used in the eradication of systemic pathogens, conservation and international transference cassava germoplasm. In this work a descriptor of growth and development evaluation of cassava accessions was proposed, indicating that 80 and 95% of the meristems evaluated in the culture medium 4E and plantlets in the culture medium 8S, respectively, achieved normal to vigorous growth. In the international germplasm transference 278 accesions were transferred from UNPRG to CIAT and 249 accesions from CIAT to UNPRG.
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Flores-Meza, M. M. (2014). In vitro clonal propagation, conservation and international transference of germplasm of cassava (Manihot esculenta Crantz) native of Peru. Revista Latinoamericana De Recursos Naturales, 10(2), 32-44. Retrieved from https://revista.itson.edu.mx/index.php/rlrn/article/view/230
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References
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Adejare G.O., Coutts R.H.A. 1981. Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture. Plant Cell Tissue and Organ Culture 1:25-32.
APG III. 2009. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Botanical Journal of the Linnean Society 161:105-121.
Barrueto Cid L.P., Carvalho LL.C.B. 2008. Importance of abscisic acid (ABA) in the in vitro conservation of cassava (Manihot esculenta Crantz). Chilean Journal of Agricultural Research 68:304-308.
Blakesley D., Pask N., Henshaw G.G., Fay M.F. 1996. Biotechnology and the conservation of forest genetic resources: In vitro strategies and cryopreservation. Plant Growth Regulation 20:11-16.
Ceballos H, De la Cruz H. 2002. Taxonomía y morfología de la yuca. En Ospina B., Ceballos H. (Comps.). La yuca en el tercer milenio. Sistemas modernos de producción, procesamiento, utilización y comercialización. CIAT. Cali, Colombia. Pp. 17-33
Cronquist A. 1988. The Evolution and Classification of Flowering Plants. Second Edition. The New York Botanical Garden, USA. 555 p.
Cruz-Cruz C.A., González-Arnao M.T., Engelmann F. 2013. Biotechnology and conservation of plant biodiversity. Resources 2:73-95.
Danso K.E., Acheampong E., Amoatey H.M. 1999. Selection and in-vitro propagation of five cassava (Manihot esculenta Crantz) cultivars. Journal of the Ghana Science Association 1:31-41.
Debouck D., Guevara C. 1995. Unidad de Recursos genéticos. Laboratorio de Cultivo de Tejidos. Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia. 16 p. (Multicopiado).
Delgado G.E., Rojas C. 1993. Cassava “seed” production program by meristem culture in UNPRG, Lambayeque, Peru. In: Proceedings of the First International Scientific Meeting, Cassava Biotechnology Network, Cartagena, Colombia, 25-28 August 1992. Thro, AM and Roca W (eds.), pp. 146-148. CIAT Working Doc. No 123, 496 p.
Engelmann F. 2011. Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology.- Plant 47:5-16.
Escobar R.H., Mafla G., Roca W.M. 1997. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports 16:474-478.
Frison E.A. 1994. Sanitation techniques for cassava. Tropical Science 34:146-153.
Frison, EA, Feliu E (1991) FAO/IBPGR technical guidelines for the safe movement of cassava germplasm. Food and Agriculture Organization of the United Nations-FAO- and International Board for Plant genetic Resources, Rome, Italy.
Fukuda W.M.G., Guevara C.L. 1998. Descritores morfológicos e agronómicos para a caracterização de mandioca (Manihot esculenta Crantz). Cruz das Almas: EMBRAPA-CNPMF, 38 p. (EMBRAPA-CNPFM. Documentos, 78).
Guo J-Y, Liu Y.O. 1995. Rapid propagation of cassava by tissue culture and its application in rural distribution in China. In Cassava Biotechnology Network: Proceedings of the 2nd International Scientific Meeting, Bogor, Indonesia. CIAT working document 150, 183-189.
Gulick P., Hershey D., Esquinas Alcazar J. 1983. Genetic resources of cassava and wild relatives. International Board for Plant Genetic Resources (IBPGR) Secretariat. 56 p.
Kaiser W.J., Teemba L.R. 1979. Use of tissue culture and thermoterapy to free East African cassava cultivars of African cassava mosaic and cassava brown streak diseases. Plant Disease Reports 63:780-784.
Kartha K.K., Gamborg O.L., Constable F., Shyluk J.P. 1974. Regeneration of cassava plants from apical meristems. Plant Science Letters 2:107-113.
Koo B., Pardey P.G., Debouck D.G. 2004. CIAT Genebank. “Saving seeds - The economics of conserving crop genetic resources ex situ in the Future Harvest Centres of the CGIAR”. Koo B, Pardey PG & Wright BD (eds.), CABI Publishing, Wallingford, United Kingdom. Pp. 105-125.
Konan N.K., Sangwan R.S., Sangwan-Norreel B.S. 1994. Efficient in vitro shoot regeneration systems in cassava (Manihot esculenta Crantz). Plant Breeding 113:227-236.
Konan N.K., Schöpke C., Cárcamo R., Beachy R.N., Fauquet C. 1997. An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems. Plant Cell Reports 16:444-449.
Mabanza J., Rodríguez-Andriyaniasi A.V., Mahouka J., Boumba B. 1995. Evaluation of cleaned cassava varieties in Congo. In: The cassava biotechnology network. Proceedings of the Second International Scientific Meeting, Bogor, Indonesia, 22-26 Agust 1994. Vol. 1, pp. 194-201. CIAT, Cali.
Mafla G., Roa J.C., Guevara C.L. 2000. Advances on the in vitro growth control of cassava using silver nitrate. In: Carvalho L.J.C.B., Thro A.M., Vilarinhos A.D., eds. Proceedings IV International Scientific Meeting of the Cassava Biotechnology Network, Salvador, Bahia, Brazil. November 03-07, 1998. EMBRAPA, CENARGEN and CBN. Brasilia, Brazil. Pp 439-446.
Mafla G., Roa J.C., Aranzales E., Debouck D.G. 2007. Manual de procedimientos para la conservación in vitro del germoplasma del género Manihot. Centro Internacional de Agricultura Tropical, Cali, Colombia. 50 p.
Mapayi E.F., Ojo D.K., Oduwaye O.A., Porbeni J.B. 2013. Optimization of in-vitro propagation of cassava (Manihot esculenta Crantz) genotypes. Journal of Agricultural Science 5:261-269.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.
Ogero K.O., Mburugu G.N., Mwangi M., Ombori O., Ngugi M. 2012. In vitro micropropagation of cassava through low cost tissue culture. Asian Journal of Agricultural science 4:205-209.
Puonti-Kaerlas J. 1998. Cassava Biotechnology. In: Biotechnology Genetic Engineering Reviews 15:330-364. Taylor and Francis Group.
Roca W.M. 1980. El cultivo de meristemas de yuca. Guía de estudio. Centro Internacional de Agricultura Tropical, Cali, Colombia. 40 p.
Roca W.M. 1985. Conservación in vitro. Lecturas sobre Recursos Fitogenéticos No 3. Consejo Internacional de Recursos Fitogenéticos (CIRF), Cali, Colombia. 46 p.
Roca W.M., Chávez R., Marin M.L., Arias D.I., Mafla G., Reyes R. 1989. In vitro methods of germplasm conservation. Genome 31:813-817.
Shahin E.A., Shepard J.F. 1980. Cassava mesophyll protoplast: isolation, proliferation and shoot formation. Plant Science Letters 17:459-465.
Smith M.K., Biggs B.J., Scott K.J. 1986. In vitro propagation of cassava (Manihot esculenta Crantz). Plant Cell, Tissue and Organ Culture 6:221-228.
Suárez L., Hernández M.M. 2008. Efecto de una mezcla de oligogalacturónidos en la propagación in vitro de la yuca (Manihot esculenta Crantz), var. CMC-40. Cultivos Tropicales 29:47-52.
Thro A.M., Roca W., Iglesias C., Henry G., Ng S.Y.C. 1998. Contributions of in-vitro biology to cassava improvement. African Crop Science Journal 6:303-315.
Tilquin J.P. 1979. Plant regeneration from stem callus of cassava. Canadian Journal of Botany 57:1761-1763.
Wasswa P., Alicai T., Mukasa S.B. 2010. Optimization of in vitro techniques for cassava brown streak virus elimination from infected cassava clones. African Crop Science Journal 18:235-241.
Adejare G.O., Coutts R.H.A. 1981. Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture. Plant Cell Tissue and Organ Culture 1:25-32.
APG III. 2009. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Botanical Journal of the Linnean Society 161:105-121.
Barrueto Cid L.P., Carvalho LL.C.B. 2008. Importance of abscisic acid (ABA) in the in vitro conservation of cassava (Manihot esculenta Crantz). Chilean Journal of Agricultural Research 68:304-308.
Blakesley D., Pask N., Henshaw G.G., Fay M.F. 1996. Biotechnology and the conservation of forest genetic resources: In vitro strategies and cryopreservation. Plant Growth Regulation 20:11-16.
Ceballos H, De la Cruz H. 2002. Taxonomía y morfología de la yuca. En Ospina B., Ceballos H. (Comps.). La yuca en el tercer milenio. Sistemas modernos de producción, procesamiento, utilización y comercialización. CIAT. Cali, Colombia. Pp. 17-33
Cronquist A. 1988. The Evolution and Classification of Flowering Plants. Second Edition. The New York Botanical Garden, USA. 555 p.
Cruz-Cruz C.A., González-Arnao M.T., Engelmann F. 2013. Biotechnology and conservation of plant biodiversity. Resources 2:73-95.
Danso K.E., Acheampong E., Amoatey H.M. 1999. Selection and in-vitro propagation of five cassava (Manihot esculenta Crantz) cultivars. Journal of the Ghana Science Association 1:31-41.
Debouck D., Guevara C. 1995. Unidad de Recursos genéticos. Laboratorio de Cultivo de Tejidos. Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia. 16 p. (Multicopiado).
Delgado G.E., Rojas C. 1993. Cassava “seed” production program by meristem culture in UNPRG, Lambayeque, Peru. In: Proceedings of the First International Scientific Meeting, Cassava Biotechnology Network, Cartagena, Colombia, 25-28 August 1992. Thro, AM and Roca W (eds.), pp. 146-148. CIAT Working Doc. No 123, 496 p.
Engelmann F. 2011. Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology.- Plant 47:5-16.
Escobar R.H., Mafla G., Roca W.M. 1997. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports 16:474-478.
Frison E.A. 1994. Sanitation techniques for cassava. Tropical Science 34:146-153.
Frison, EA, Feliu E (1991) FAO/IBPGR technical guidelines for the safe movement of cassava germplasm. Food and Agriculture Organization of the United Nations-FAO- and International Board for Plant genetic Resources, Rome, Italy.
Fukuda W.M.G., Guevara C.L. 1998. Descritores morfológicos e agronómicos para a caracterização de mandioca (Manihot esculenta Crantz). Cruz das Almas: EMBRAPA-CNPMF, 38 p. (EMBRAPA-CNPFM. Documentos, 78).
Guo J-Y, Liu Y.O. 1995. Rapid propagation of cassava by tissue culture and its application in rural distribution in China. In Cassava Biotechnology Network: Proceedings of the 2nd International Scientific Meeting, Bogor, Indonesia. CIAT working document 150, 183-189.
Gulick P., Hershey D., Esquinas Alcazar J. 1983. Genetic resources of cassava and wild relatives. International Board for Plant Genetic Resources (IBPGR) Secretariat. 56 p.
Kaiser W.J., Teemba L.R. 1979. Use of tissue culture and thermoterapy to free East African cassava cultivars of African cassava mosaic and cassava brown streak diseases. Plant Disease Reports 63:780-784.
Kartha K.K., Gamborg O.L., Constable F., Shyluk J.P. 1974. Regeneration of cassava plants from apical meristems. Plant Science Letters 2:107-113.
Koo B., Pardey P.G., Debouck D.G. 2004. CIAT Genebank. “Saving seeds - The economics of conserving crop genetic resources ex situ in the Future Harvest Centres of the CGIAR”. Koo B, Pardey PG & Wright BD (eds.), CABI Publishing, Wallingford, United Kingdom. Pp. 105-125.
Konan N.K., Sangwan R.S., Sangwan-Norreel B.S. 1994. Efficient in vitro shoot regeneration systems in cassava (Manihot esculenta Crantz). Plant Breeding 113:227-236.
Konan N.K., Schöpke C., Cárcamo R., Beachy R.N., Fauquet C. 1997. An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems. Plant Cell Reports 16:444-449.
Mabanza J., Rodríguez-Andriyaniasi A.V., Mahouka J., Boumba B. 1995. Evaluation of cleaned cassava varieties in Congo. In: The cassava biotechnology network. Proceedings of the Second International Scientific Meeting, Bogor, Indonesia, 22-26 Agust 1994. Vol. 1, pp. 194-201. CIAT, Cali.
Mafla G., Roa J.C., Guevara C.L. 2000. Advances on the in vitro growth control of cassava using silver nitrate. In: Carvalho L.J.C.B., Thro A.M., Vilarinhos A.D., eds. Proceedings IV International Scientific Meeting of the Cassava Biotechnology Network, Salvador, Bahia, Brazil. November 03-07, 1998. EMBRAPA, CENARGEN and CBN. Brasilia, Brazil. Pp 439-446.
Mafla G., Roa J.C., Aranzales E., Debouck D.G. 2007. Manual de procedimientos para la conservación in vitro del germoplasma del género Manihot. Centro Internacional de Agricultura Tropical, Cali, Colombia. 50 p.
Mapayi E.F., Ojo D.K., Oduwaye O.A., Porbeni J.B. 2013. Optimization of in-vitro propagation of cassava (Manihot esculenta Crantz) genotypes. Journal of Agricultural Science 5:261-269.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.
Ogero K.O., Mburugu G.N., Mwangi M., Ombori O., Ngugi M. 2012. In vitro micropropagation of cassava through low cost tissue culture. Asian Journal of Agricultural science 4:205-209.
Puonti-Kaerlas J. 1998. Cassava Biotechnology. In: Biotechnology Genetic Engineering Reviews 15:330-364. Taylor and Francis Group.
Roca W.M. 1980. El cultivo de meristemas de yuca. Guía de estudio. Centro Internacional de Agricultura Tropical, Cali, Colombia. 40 p.
Roca W.M. 1985. Conservación in vitro. Lecturas sobre Recursos Fitogenéticos No 3. Consejo Internacional de Recursos Fitogenéticos (CIRF), Cali, Colombia. 46 p.
Roca W.M., Chávez R., Marin M.L., Arias D.I., Mafla G., Reyes R. 1989. In vitro methods of germplasm conservation. Genome 31:813-817.
Shahin E.A., Shepard J.F. 1980. Cassava mesophyll protoplast: isolation, proliferation and shoot formation. Plant Science Letters 17:459-465.
Smith M.K., Biggs B.J., Scott K.J. 1986. In vitro propagation of cassava (Manihot esculenta Crantz). Plant Cell, Tissue and Organ Culture 6:221-228.
Suárez L., Hernández M.M. 2008. Efecto de una mezcla de oligogalacturónidos en la propagación in vitro de la yuca (Manihot esculenta Crantz), var. CMC-40. Cultivos Tropicales 29:47-52.
Thro A.M., Roca W., Iglesias C., Henry G., Ng S.Y.C. 1998. Contributions of in-vitro biology to cassava improvement. African Crop Science Journal 6:303-315.
Tilquin J.P. 1979. Plant regeneration from stem callus of cassava. Canadian Journal of Botany 57:1761-1763.
Wasswa P., Alicai T., Mukasa S.B. 2010. Optimization of in vitro techniques for cassava brown streak virus elimination from infected cassava clones. African Crop Science Journal 18:235-241.