Large-scale propagation of matico (Piper tuberculatum Jacq.) for the purpose of eradication of metaxenic diseases vectors
Main Article Content
Abstract
The abusive and uncontrolled use of insecticides for the control of vectors of diseases such as dengue and malaria, Leishmaniasis and Trypanosomiasis have caused irreparable damage to the environment, by the waste in the soil and water, food poisoning and creating resistance in the insects, resulting in more difficult eradication of diseases that are transmitted in humans. For this reason, the objective of the present study was to evaluate the in vitro mass propagation of ‘matico’ (Piper tuberculatum) and its application in the control of vectors of metaxenic diseases in Lambayeque, a region on the north coast of Peru with increasing incidence of dengue, transmitted by Aedes aegypti and Leishmaniasis (“uta”), transmitted by Lutzomya sp. P. tuberculatum is a species that contains numerous secondary metabolites like amides, lignans and flavonoids, with great insecticidal activity. The results indicated that using the in vitro culture of shoot apices and nodal segments it is possible to obtain thousands of plants that can be successfully transferred to field conditions and produce extracts of inflorescences with insecticidal potential. The results indicate that using the in vitro culture of shoot apices and nodal segments, thousands of plants are obtained that can be transferred successfully to field conditions and by means of an extraction process with organic solvents, obtain secondary metabolites with bioinsecticidal activity.
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Delgado-Paredes, G. E. (2017). Large-scale propagation of matico (Piper tuberculatum Jacq.) for the purpose of eradication of metaxenic diseases vectors. Revista Latinoamericana De Recursos Naturales, 13(2), 39-50. Retrieved from https://revista.itson.edu.mx/index.php/rlrn/article/view/263
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References
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Aloísio X. 1997. Enraizamiento in vitro de gemas de Eucalyptus multiplicados e elongados. Revista Scientia Forestalis. 51:26-29.
Agramonte D, Jiménez F, Dita MA. 1998. Aclimatización. En: Pérez Ponce JN (ed.). Propagación y Mejora Genética de Plantas por Biotecnología. Instituto de Biotecnología de Plantas. Santa Clara, Villa Clara, Cuba, pp. 193-206.
APG IV. 2016. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants. Botanical Journal of the Linnean Society 181:1-20.
Bacon C.W., White J.F. 2000. Microbial endophytes. Marcel Dekker Inc., New York, N.Y.
Balbuena T., Santa-Catarina C., Silveira V., Kato M.J., Floh E.I.S. 2009. In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C.DC. (Piperaceae). Acta Botánica Brasileira. 23:274-281.
Bazán-Calderón J., Ventura-Flores R., Kato M.J., Rojas-Idrogo, C., Delgado-Paredes G.E. 2011. Actividad insecticida de Piper tuberculatum Jacq. sobre Aedes aegypti L. (Diptera: Culicidae) y Anopheles pseudopunctipennis Tehobal (Diptera: Culicidae). Anales de Biología 33:135-147.
Barbedo C.J., Centeno D.C., Ribeiro R.C.L.F. 2013. Do recalcitrant seeds really exist?. Hoehnea 40:583-593.
Bhat S.R., Chandel K.P.S., Malik S.K. 1995. Plant regeneration from various explants of cultivated Piper species. Plant Cell Reports 14:398-402.
Berjak P., Pammentar N.W. 2013. Implications of the lack of desiccation tolerance in recalcitrat seeds. Frontiers in Plant Science 4, article 478 1-9.
Brako L, Zarucchi JL. Catalogue of the Flowering Plants and gymnosperms of Peru. 1993. Monographs in Systematic Botany from the Missouri Botanical Garden. Vol. 45.
Cotinguiba F., Regasini L.O., Bolzani V.S., Debonsi H.M., Passerini G.D., Cicarelli R.M.B., Kato M.J., Furlan M. 2009. Piperamides and their derivatives as potential anti-trypanosomal agents. Medicinal Chemistry Research 18:703-711.
Danelutte A.P., Lago J.H.G., Young M.C.M., Kato M.J. 2003. Antifungal flavanones and prenylated hydroquinones from Piper crassinervium Kunth. Phytochemistry 64:555-559. FALTA?
Delgado-Paredes G.E., Kato M.J., Vásquez-Dueñas N., Minchala-Patiño J., Rojas-Idrogo C. 2012. Cultivo de tejidos de Piper sp. (Piperaceae): Propagación, organogénesis y conservación de germoplasma in vitro. Revista Colombiana de Biotecnología 14:49-60.
Díaz LP, Medina LF, Latife J, Digonzelli PA, Sosa SB. 2004. Aclimatación de plantas micropropagadas de caña de azúcar utilizando el humus de lombriz. RIA 33:115-128.
Dominguez F., Lozoya X., Simon J. 2006. Tissue culture regeneration of a medicinal plant from Mexico: Piper auritum Kunth. HortScience 41:207-209.
GERESA (Gerencia Regional de Salud – Lambayeque). 2017. Boletín Epidemiológico S.E. 36-2017. (Lambayeque, Perú). 20 p.
Hussain A., Naz S., Nazir H., Khan Z. 2011. Tissue culture of black pepper (Piper nigrum L.) in Pakistan. Pakistan Journal of Botany 43:1069-1078.
Jaramillo M.A., Manos P.S., Zimmer E.A. 2004. Phylogenetic relationships of the perianthless piperales: reconstructing the evolution of floral development. International Journal of Plant Science 165:403-416.
Kato M.J., Furlan M. 2007. Chemistry and evolution of Piperaceae. Pure and Applied Chemistry 79:529-538.
Kelkar S.M., Krishnamurthy K.V. 1998. Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. Plant Cell Reports 17:721-725.
Kusari S., Hertweck C., Spiteller M. 2012. Chemical ecology of endophytic fungi: origins of secondary metabolites. Chemistry & Biology 19:792-798.
Lago J.H.G., Ramos C.S., Casanova D.C.C., Morandim A.A., Bergamo D.C.B., Cavalheiro A.J., Bolzani V.S., Furlan M., Guimarães E.F., Young M.C.M., Kato M.J. 2004. Benzoic acid derivatives from Piper species and their fungitoxic activity against Cladosporium cladosporioides and C. sphaerospermum. Journal of Natural Products 67:1783-1788. FALTA?
López E.A., Durán J., Cuervo R. 2010. Extracción y formulación de un bioinsecticida a partir de amida isobutílica 4,5-dihydro-piperlonguminina extraída del pipilongo (Piper tuberculatum) para el control del pulgón amarillo (Sipha flava) en caña de azúcar (Saccharum officinarum). Revista Científica Guillermo de Ockham 8:115-123.
Ludlow B., Vásquez-Yañes C. 1979. Germinación de semillas de Piper hispidum Sw. bajo diferentes condiciones de iluminación. En: Investigaciones sobre la Regeneración de Selvas altas en Veracruz, México. Gómez Pompa A., Del Amo S., Vázquez-Yanes C. y Butanda A. (eds.). 2da. Ed. México: Compañía Editorial Continental, S.A., p. 263-277.
Mabberley D.J. 1997. The Plant Book. A Portable Dictionary of the Higher Plants. New York, USA: Cambridge University Press.
Martínez-Ruiz R., Azpíroz-Rivero H.S., Rodríguez-De la O J.L., Cetina-Alcalá V.M., Gutiérrez-Espinosa M.A. 2005. Aclimatación de plantas obtenidas in vitro de Eucalyptus urophylla ST Blake y Eucalyptus grandis Hill ex Maiden Ra Ximhai 1:591-597.
Mathews V.H., Rao P.S. 1984. In vitro responses in black pepper (Piper nigrum). Current Science 53:183-186.
Medina L.F., Jaime M., Chueca C., Bocanegra B., Toro F., Mascaró P. 2001. Presencia y cuantificación de Azotobacter sp. y Azospirillum sp. en lombricompuesto. Segunda Reunión de Producción Vegetal del NOA. San Miguel de Tucumán, Argentina.
Mendoza-Frías N.L., Monsalve-Asencio L.J., Rojas-Idrogo C., Kato M.J., Delgado-Paredes G.E. 2013. Insecticidal activity of Piper tuberculatum extracts on the cotton staigner bug, Dysdercus peruvianus Guérin-Méneville (Hemiptera: Pyrrhocoridae). Academic Journal of Entomology 6:153-161.
Miyakado M., Nakayama I., Ohno N. 1989. Insecticidal unsaturated isobutylamides: From natural products to agrochemical leads. In: Arnason J.T., Philogène B.J.R., Morand P. (eds.). Insecticides of Plant Origin. ACS Symposium Series 387, American Chemical Society, New York. Pp. 183-187.
Monsalve-Asencio L., Mendoza-Frías N.L., Rojas-Idrogo C., Kato M.J., Saavedra-Díaz, J., Delgado-Paredes, G.E. 2015. Larvicidal activity of Piper tuberculatum extracts on the tobacco budworm, Heliothis virescens Fabr. (Lepidoptera:Noctuidae) under laboratory conditions. International Journal of Pure & Applied Bioscience 3:1-9.
Murashige T. 1974. Plant propagation through tissue culture. Annual Review of Plant Physiology 25:135-166.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.
Navickiene H.M.D., Alécio A.C., Kato M.J., Bolzani V. da S., Young M.C.M., Cavalheiro A.J., Furlan M. 2000. Antifungal amides from Piper hispidum and Piper tuberculatum. Phytochemistry 55:621-626.
Navickiene H.M.F., Miranda J.E., Bortoli S.A., Kato M.J., Bolzani V da S., Furlan M. 2007. Toxicity of extracts and isobutyl amides from Piper tuberculatum: potent compounds with potential for the control of the veltbean caterpillar, Anticarsia gemmatalis. Pest Management Science 63:399-403.
Ntonifor N.N. 2011. Potentials of tropical African species as sources of reduced-risk pesticides. Journal of Entomology 8:16-26.
Palacios Z.G.F., Delgado G.E., Moreno M.C., Kato M.J., Rojas C. 2009. Actividad antifúngica in vitro de extractos crudos de Piper tuberculatum. Revista Peruana de Biología 16:209-214.
Parmar V.S., Jain S.C., Bisht K.S., Jain R., Taneja P., Jha A., Tyagi O.D., Prasad A.K., Wengel J., Olsen C.E., Boll P.M. 1997. Phytochemistry of the genus Piper. Phytochemistry 47:597-673.
Parmar V.S., Jain S.C., Gupta S., Talwar S., Rajwanshi V.K., Kumar R., Azim A., Malhotra S., Kumar N., Jain R., Sharma N.K., Tyagi O.D., Lawrie S.J., Errington W., Howarth O.W., Olsen C.E., Singh S.K., Wengel J. 1998. Polyphenols and alkaloids from Piper species. Phytochemistry 49:1069-1078.
Preece JE, Sutter EG. 1991. Acclimatization of micropropagated plants to greenhouse and field. In: Debergh PC, Zimmerman RH (eds.). Micropropagation: Technology and Application. Kluwer Academic, Dordrecht, pp. 71-93.
Raj L., Ide T., Gurkar A.U., Foley M., Schenone M., Li X., Tolliday N.J., Golub T.R., Carr S.A., Shamji A.F., Stern A.M., Mandinova A., Schreiber S.L., Lee S.W. 2011. Selective killing of cancer cells with a small molecule targeting stress response to ROS. Nature 475:231-234.
Regasini L.O., Cotinguiba F., Morandim A.A., Kato M.J., Scorzoni L., Mendes-Giannini M.J., Bolzani V. da S., Furlan M. 2009. Antimicrobial cativity of Piper arboreum and Piper tuberculatum (Piperaceae) against opportunistic yeasts. African Journal of Biotechnology 8:2866-2870.
Richards L.A., Dyer L.A., Forister M.L., Smilanch A.M., Dodson C.D., Leonard M.D., Jeffrey C.S. 2015. Phytochemical diversity drives plant-insect community diversity. Proceedings of the National Academic of Sciences 112:10973-10978.
Roberts E.H. 1973. Predicting the storage life of seeds. Seed Science and Technology 1:499-514.
Schultes R.F., Raffauf R.F. 1990. Medicinal and toxic plants of the Indians of northwest Amazonia, Piperaceae. In: Schultes R.E., Raffauf R.F. (eds.). The Healling Forest: Medicinal and Toxic Plants of the Northwest Amazonia. Historical, Etho- & Economic Botany Series. Vol. 2. Dioscoride Press, Portland, Oregon. Pp. 362-368.
Scott L.M., Helson B.V., Strunz G.M., Finlay H. 2007. Efficacy of Piper nigrum (Piperaceae) extract for control of insect defoliators of forest and ornamental trees. The Canadian Entomologist 139:513-522.
Silva R.V., Navickiene H.M.D., Kato M.J., Bolzani V., Méda C.I., Young M.C.M., Furlan M. 2002. Antifungal amides from Piper arboreum and Piper tuberculatum. Phytochemistry 59: 521-527. FALTA?
Soberón G.V., Rojas C., Saavedra J., Kato M.J., Delgado G.E. 2006. Acción biocida de plantas de Piper tuberculatum Jacq. sobre Diatraea saccharalis (Lepidoptera, Pyralidae). Revista Peruana de Biología 13:107-112.
Soberón G.V., Rojas Idrogo C., Kato M.J., Saavedra Díaz J., Armando Jr. J., Delgado Paredes G.E. 2012. Larvicidal activity of Piper tuberculatum on Spodoptera frugiperda (Lepidoptera: Noctuidae) under laboratory conditions. Revista Colombiana de Entomología 38:35-41.
Taylor D.W., Hickey L.J. 1992. Phylogenetic evidence for the herbaceous origin of angiosperm. Plant Systematic and Evolution. 180:137-156.
Tebbs M.C. 1989. Revision of Piper (Piperaceae) in the New World. 1. Review of characters and taxonomy of Piper section Macrostachys. Bulletin of the Natural History Museum of London 19:117-158.
Trelease W. 1936. Piperaceae. In: Flora of Peru, Vol. XIII. MacBride J.F. (ed.). Chicago, USA. Field Museum of Natural History, 253 pp.
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Ahmed ABA, Mohajer S, Elnaiem EM, Taha RM. 2012. In vitro regeneration, acclimatization and antimicrobial studies of selected ornamental plants. Chapter 11. Plant Science. Downloaded from: http://www.intechopen.com/books/plant-science
Aloísio X. 1997. Enraizamiento in vitro de gemas de Eucalyptus multiplicados e elongados. Revista Scientia Forestalis. 51:26-29.
Agramonte D, Jiménez F, Dita MA. 1998. Aclimatización. En: Pérez Ponce JN (ed.). Propagación y Mejora Genética de Plantas por Biotecnología. Instituto de Biotecnología de Plantas. Santa Clara, Villa Clara, Cuba, pp. 193-206.
APG IV. 2016. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants. Botanical Journal of the Linnean Society 181:1-20.
Bacon C.W., White J.F. 2000. Microbial endophytes. Marcel Dekker Inc., New York, N.Y.
Balbuena T., Santa-Catarina C., Silveira V., Kato M.J., Floh E.I.S. 2009. In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C.DC. (Piperaceae). Acta Botánica Brasileira. 23:274-281.
Bazán-Calderón J., Ventura-Flores R., Kato M.J., Rojas-Idrogo, C., Delgado-Paredes G.E. 2011. Actividad insecticida de Piper tuberculatum Jacq. sobre Aedes aegypti L. (Diptera: Culicidae) y Anopheles pseudopunctipennis Tehobal (Diptera: Culicidae). Anales de Biología 33:135-147.
Barbedo C.J., Centeno D.C., Ribeiro R.C.L.F. 2013. Do recalcitrant seeds really exist?. Hoehnea 40:583-593.
Bhat S.R., Chandel K.P.S., Malik S.K. 1995. Plant regeneration from various explants of cultivated Piper species. Plant Cell Reports 14:398-402.
Berjak P., Pammentar N.W. 2013. Implications of the lack of desiccation tolerance in recalcitrat seeds. Frontiers in Plant Science 4, article 478 1-9.
Brako L, Zarucchi JL. Catalogue of the Flowering Plants and gymnosperms of Peru. 1993. Monographs in Systematic Botany from the Missouri Botanical Garden. Vol. 45.
Cotinguiba F., Regasini L.O., Bolzani V.S., Debonsi H.M., Passerini G.D., Cicarelli R.M.B., Kato M.J., Furlan M. 2009. Piperamides and their derivatives as potential anti-trypanosomal agents. Medicinal Chemistry Research 18:703-711.
Danelutte A.P., Lago J.H.G., Young M.C.M., Kato M.J. 2003. Antifungal flavanones and prenylated hydroquinones from Piper crassinervium Kunth. Phytochemistry 64:555-559. FALTA?
Delgado-Paredes G.E., Kato M.J., Vásquez-Dueñas N., Minchala-Patiño J., Rojas-Idrogo C. 2012. Cultivo de tejidos de Piper sp. (Piperaceae): Propagación, organogénesis y conservación de germoplasma in vitro. Revista Colombiana de Biotecnología 14:49-60.
Díaz LP, Medina LF, Latife J, Digonzelli PA, Sosa SB. 2004. Aclimatación de plantas micropropagadas de caña de azúcar utilizando el humus de lombriz. RIA 33:115-128.
Dominguez F., Lozoya X., Simon J. 2006. Tissue culture regeneration of a medicinal plant from Mexico: Piper auritum Kunth. HortScience 41:207-209.
GERESA (Gerencia Regional de Salud – Lambayeque). 2017. Boletín Epidemiológico S.E. 36-2017. (Lambayeque, Perú). 20 p.
Hussain A., Naz S., Nazir H., Khan Z. 2011. Tissue culture of black pepper (Piper nigrum L.) in Pakistan. Pakistan Journal of Botany 43:1069-1078.
Jaramillo M.A., Manos P.S., Zimmer E.A. 2004. Phylogenetic relationships of the perianthless piperales: reconstructing the evolution of floral development. International Journal of Plant Science 165:403-416.
Kato M.J., Furlan M. 2007. Chemistry and evolution of Piperaceae. Pure and Applied Chemistry 79:529-538.
Kelkar S.M., Krishnamurthy K.V. 1998. Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. Plant Cell Reports 17:721-725.
Kusari S., Hertweck C., Spiteller M. 2012. Chemical ecology of endophytic fungi: origins of secondary metabolites. Chemistry & Biology 19:792-798.
Lago J.H.G., Ramos C.S., Casanova D.C.C., Morandim A.A., Bergamo D.C.B., Cavalheiro A.J., Bolzani V.S., Furlan M., Guimarães E.F., Young M.C.M., Kato M.J. 2004. Benzoic acid derivatives from Piper species and their fungitoxic activity against Cladosporium cladosporioides and C. sphaerospermum. Journal of Natural Products 67:1783-1788. FALTA?
López E.A., Durán J., Cuervo R. 2010. Extracción y formulación de un bioinsecticida a partir de amida isobutílica 4,5-dihydro-piperlonguminina extraída del pipilongo (Piper tuberculatum) para el control del pulgón amarillo (Sipha flava) en caña de azúcar (Saccharum officinarum). Revista Científica Guillermo de Ockham 8:115-123.
Ludlow B., Vásquez-Yañes C. 1979. Germinación de semillas de Piper hispidum Sw. bajo diferentes condiciones de iluminación. En: Investigaciones sobre la Regeneración de Selvas altas en Veracruz, México. Gómez Pompa A., Del Amo S., Vázquez-Yanes C. y Butanda A. (eds.). 2da. Ed. México: Compañía Editorial Continental, S.A., p. 263-277.
Mabberley D.J. 1997. The Plant Book. A Portable Dictionary of the Higher Plants. New York, USA: Cambridge University Press.
Martínez-Ruiz R., Azpíroz-Rivero H.S., Rodríguez-De la O J.L., Cetina-Alcalá V.M., Gutiérrez-Espinosa M.A. 2005. Aclimatación de plantas obtenidas in vitro de Eucalyptus urophylla ST Blake y Eucalyptus grandis Hill ex Maiden Ra Ximhai 1:591-597.
Mathews V.H., Rao P.S. 1984. In vitro responses in black pepper (Piper nigrum). Current Science 53:183-186.
Medina L.F., Jaime M., Chueca C., Bocanegra B., Toro F., Mascaró P. 2001. Presencia y cuantificación de Azotobacter sp. y Azospirillum sp. en lombricompuesto. Segunda Reunión de Producción Vegetal del NOA. San Miguel de Tucumán, Argentina.
Mendoza-Frías N.L., Monsalve-Asencio L.J., Rojas-Idrogo C., Kato M.J., Delgado-Paredes G.E. 2013. Insecticidal activity of Piper tuberculatum extracts on the cotton staigner bug, Dysdercus peruvianus Guérin-Méneville (Hemiptera: Pyrrhocoridae). Academic Journal of Entomology 6:153-161.
Miyakado M., Nakayama I., Ohno N. 1989. Insecticidal unsaturated isobutylamides: From natural products to agrochemical leads. In: Arnason J.T., Philogène B.J.R., Morand P. (eds.). Insecticides of Plant Origin. ACS Symposium Series 387, American Chemical Society, New York. Pp. 183-187.
Monsalve-Asencio L., Mendoza-Frías N.L., Rojas-Idrogo C., Kato M.J., Saavedra-Díaz, J., Delgado-Paredes, G.E. 2015. Larvicidal activity of Piper tuberculatum extracts on the tobacco budworm, Heliothis virescens Fabr. (Lepidoptera:Noctuidae) under laboratory conditions. International Journal of Pure & Applied Bioscience 3:1-9.
Murashige T. 1974. Plant propagation through tissue culture. Annual Review of Plant Physiology 25:135-166.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.
Navickiene H.M.D., Alécio A.C., Kato M.J., Bolzani V. da S., Young M.C.M., Cavalheiro A.J., Furlan M. 2000. Antifungal amides from Piper hispidum and Piper tuberculatum. Phytochemistry 55:621-626.
Navickiene H.M.F., Miranda J.E., Bortoli S.A., Kato M.J., Bolzani V da S., Furlan M. 2007. Toxicity of extracts and isobutyl amides from Piper tuberculatum: potent compounds with potential for the control of the veltbean caterpillar, Anticarsia gemmatalis. Pest Management Science 63:399-403.
Ntonifor N.N. 2011. Potentials of tropical African species as sources of reduced-risk pesticides. Journal of Entomology 8:16-26.
Palacios Z.G.F., Delgado G.E., Moreno M.C., Kato M.J., Rojas C. 2009. Actividad antifúngica in vitro de extractos crudos de Piper tuberculatum. Revista Peruana de Biología 16:209-214.
Parmar V.S., Jain S.C., Bisht K.S., Jain R., Taneja P., Jha A., Tyagi O.D., Prasad A.K., Wengel J., Olsen C.E., Boll P.M. 1997. Phytochemistry of the genus Piper. Phytochemistry 47:597-673.
Parmar V.S., Jain S.C., Gupta S., Talwar S., Rajwanshi V.K., Kumar R., Azim A., Malhotra S., Kumar N., Jain R., Sharma N.K., Tyagi O.D., Lawrie S.J., Errington W., Howarth O.W., Olsen C.E., Singh S.K., Wengel J. 1998. Polyphenols and alkaloids from Piper species. Phytochemistry 49:1069-1078.
Preece JE, Sutter EG. 1991. Acclimatization of micropropagated plants to greenhouse and field. In: Debergh PC, Zimmerman RH (eds.). Micropropagation: Technology and Application. Kluwer Academic, Dordrecht, pp. 71-93.
Raj L., Ide T., Gurkar A.U., Foley M., Schenone M., Li X., Tolliday N.J., Golub T.R., Carr S.A., Shamji A.F., Stern A.M., Mandinova A., Schreiber S.L., Lee S.W. 2011. Selective killing of cancer cells with a small molecule targeting stress response to ROS. Nature 475:231-234.
Regasini L.O., Cotinguiba F., Morandim A.A., Kato M.J., Scorzoni L., Mendes-Giannini M.J., Bolzani V. da S., Furlan M. 2009. Antimicrobial cativity of Piper arboreum and Piper tuberculatum (Piperaceae) against opportunistic yeasts. African Journal of Biotechnology 8:2866-2870.
Richards L.A., Dyer L.A., Forister M.L., Smilanch A.M., Dodson C.D., Leonard M.D., Jeffrey C.S. 2015. Phytochemical diversity drives plant-insect community diversity. Proceedings of the National Academic of Sciences 112:10973-10978.
Roberts E.H. 1973. Predicting the storage life of seeds. Seed Science and Technology 1:499-514.
Schultes R.F., Raffauf R.F. 1990. Medicinal and toxic plants of the Indians of northwest Amazonia, Piperaceae. In: Schultes R.E., Raffauf R.F. (eds.). The Healling Forest: Medicinal and Toxic Plants of the Northwest Amazonia. Historical, Etho- & Economic Botany Series. Vol. 2. Dioscoride Press, Portland, Oregon. Pp. 362-368.
Scott L.M., Helson B.V., Strunz G.M., Finlay H. 2007. Efficacy of Piper nigrum (Piperaceae) extract for control of insect defoliators of forest and ornamental trees. The Canadian Entomologist 139:513-522.
Silva R.V., Navickiene H.M.D., Kato M.J., Bolzani V., Méda C.I., Young M.C.M., Furlan M. 2002. Antifungal amides from Piper arboreum and Piper tuberculatum. Phytochemistry 59: 521-527. FALTA?
Soberón G.V., Rojas C., Saavedra J., Kato M.J., Delgado G.E. 2006. Acción biocida de plantas de Piper tuberculatum Jacq. sobre Diatraea saccharalis (Lepidoptera, Pyralidae). Revista Peruana de Biología 13:107-112.
Soberón G.V., Rojas Idrogo C., Kato M.J., Saavedra Díaz J., Armando Jr. J., Delgado Paredes G.E. 2012. Larvicidal activity of Piper tuberculatum on Spodoptera frugiperda (Lepidoptera: Noctuidae) under laboratory conditions. Revista Colombiana de Entomología 38:35-41.
Taylor D.W., Hickey L.J. 1992. Phylogenetic evidence for the herbaceous origin of angiosperm. Plant Systematic and Evolution. 180:137-156.
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