Molecular identification of pathogenic free-living amoebae in various natural and artificial water bodies in the state of Chihuahua, Mexico. First part.
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Abstract
Free-living amoebae (AVL) are protozoa widely distributed in nature, and some of them are capable of causing serious infections in humans and animals. The genera Naegleria, Acanthamoeba, Balamuthia, Sappinia, Vermamoeba, Vahlkampfia y Paravahlkampfia, have gained relevance as they have species that behave as pathogenic and opportunistic parasites. To generate information on the geographic distribution and genetic diversity of pathogenic free-living amoebae in the northern region of Mexico, the findings of the sampling of various natural and artificial aquatic environments carried out in the state of Chihuahua are presented, particularly in the near the city of Chihuahua and Ciudad Juarez. Fourteen water samples were taken in one-liter containers, seven samples from the city of Chihuahua and its surroundings, and another seven samples from Ciudad Juarez and its suburbs, including the Rio Grande. Aliquots of 50 mL were duplicated after shaking the sampling bottle and centrifuged to concentrate the sample. Pellets were seeded on non-nutritive agar plates spiked with Escherichia coli and seeded at 37 and 42°C as isolation temperatures. 98 strains were isolated, 50 at 37°C and 48 at 42°C, keeping a total of 84 strains in culture. Once the morphological identification and PCR tests were carried out, N. fowleri, Naegleria spp., Acanthamoeba spp., and other genera of uncertain identity were confirmed, which amplified with the universal primers and are awaiting the result of the sequencing. The presence of free-living pathogenic amoebas in the North and South of Chihuahua and unrelated aquatic environments that may be a source of contamination for diseases caused by free-living pathogenic amoebas is confirmed. contamination for diseases caused by free-living pathogenic amoebas is confirmed
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