Tolerance of fecal samples to different refrigeration times for the recovery of infective Haemonchus contortus larvae in coproparasitologic bioassays with sheeps
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Abstract
The collection of viable infective larvae is essential for identification of genera and species in research or parasitological diagnosis. The objective of this study was to determine the tolerance time for the preservation of fresh feces for the recovery of third stage infective larvae (L3) of Haemonchus contortus. Three trials were performed on parasite-positive sheep feces at different refrigeration times (5°C): 4d (RFG4d), 21d (RFG21d) and 42d (RFG42d). In each trial at day zero (unrefrigerated) samples were processed and was the control group (CTRL). The number of eggs per gram of feces (HGH) was determined by the McMaster technique. Subsequently, coproculture and Baerman technique were performed. The number of L3 of H. contortus per milliliter was identified. Larval emergence (Em) was calculated as Em= L3/HGH. The percentage recovery (P<0.05) of L3 and final EM was 19.99, 0.62, 0% and 36.62, 0.46, 0% in RFG4d, RFG21d and RFG42d respectively (P<0.05). A higher percentage of L3 emergence of H. contortus was obtained by stool culture with non-refrigerated feces. If the sample was not processed on the same day, it could be refrigerated for up to seven days with sufficient emergence (16.73%) to identify the genus and species of larvae.
seven days with sufficient emergence (16.73%) to identify the genus and species of larvae.
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