Bromatological and phytochemical analysis of Thalassia testudinum powder
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Published:
Aug 26, 2025
Keywords:
polyphenols, antioxidants, sustainable development
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Abstract
Thalassia testudinum is a marine plant containing bioactive antioxidant compounds and nutritional components that may enhance animal health and productivity when used as a dietary supplement. Additionally, it has potential applications as a raw material for the production of organic fertilizers aimed at improving soil fertility and promoting sustainable development. This study aimed to characterize the bromatological and phytochemical profile of T. testudinum powder for its prospective use in agroindustrial applications. Proximate analysis was performed to quantify macronutrient content, while phytochemical assays quantified flavonoids, tannins, and total phenolic compounds. Antioxidant capacity was assessed using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and hydrogen peroxide reduction assays. The powder exhibited a suitable nutritional profile, with a total phenolic content of 63.46 mg gallic acid equivalents (GAE)/g dry weight and significant antioxidant activity, evidenced by a ferric ion reduction of 339 μmol/g extract. These results suggest potential utility as both a feed additive and organic fertilizer; however, dose optimization and in vivo validation studies are necessary to confirm efficacy and safety.
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Mex-Álvarez, R. M. de J., Guillen-Morales, M. M., Uc-Guerra, J. A., Ortiz-Ballina, F. G., Chan-Martínez, R. E., & Euan-García, D. E. (2025). Bromatological and phytochemical analysis of Thalassia testudinum powder. Revista Latinoamericana De Recursos Naturales, 21(2), 76-83. Retrieved from https://revista.itson.edu.mx/index.php/rlrn/article/view/363
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References
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Bhatti, M.Z., Ali, A., Ahmad, A., Saeed, A., Malik, S.A. 2015. Antioxidant and phytochemical analysis of Ranunculus arvensis L. extracts. BMC research notes, 8, 1-8.
Buesa, R.J. 2022. Composición química de la seiba (Thalassia testudinum KD Koenig, 1805). Revista de investigaciones marinas, 42(2), 1-20. https://doi.org/10.5281/zenodo.7407414
Cabello-Pasini, A., Muñiz-Salazar, R., Ward, D.H. 2004. Caracterización bioquímica del pasto marino Zostera marina en el límite sur de su distribución en el Pacífico Norte. Ciencias marinas, 30(1A), 21-34.
Coria-Monter, E., Durán-Campos, E. 2015. Proximal analysis of seagrass species from Laguna de Términos, México. Hidrobiológica, 25(2), 249-255. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0188-88972015000200249&lng=es&tlng=
Cosme, F., Aires, A., Pinto, T., Oliveira, I., Vilela, A., Gonçalves, B. 2025. A Comprehensive Review of Bioactive Tannins in Foods and Beverages: Functional Properties, Health Benefits, and Sensory Qualities. Molecules (Basel, Switzerland), 30(4), 800. https://doi.org/10.3390/molecules30040800
Díaz, J.M., Barrios, L.M., Gómez, D.I. 2003. Fishing for life (also known as improving local management of tropical coastal resources in the face of climate change for economic wellbeing of local and vulnerable communities). Monitoreo de la biodiversidad y los ecosistemas marinos y costeros. doi:10.13140/2.1.3025.0566
Drețcanu, G., Știrbu, I., Leoplold, N., Cruceriu, D., Danciu, C., Stănilă, A., Fărcaș, A., Borda, I.M., Iuhas, C., Diaconeasa,
Z. 2022. Chemical Structure, Sources and Role of Bioactive Flavonoids in Cancer Prevention: A Review. Plants (Basel, Switzerland), 11(9), 1117. https://doi.org/10.3390/plants11091117
Durán-Lengua, M., Gaitán-Ibarra, R., Olivero-Verbel, J.T. 2013. Búsqueda en bases de datos de actividad biológica de moléculas quinoides. Revista Cubana de Información en Ciencias de la Salud, 24(4), 416-430 http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S2307-21132013000400005&lng=es&tlng=es
García-Granados, R.U., Cruz-Sosa, F., Alarcón-Aguilar, F.J., Nieto-Trujillo, A., Gallegos-Martínez, M.E. 2020. Análisis fitoquímico cualitativo de los extractos acuosos de Thalassia testudinum Banks ex Köning et Sims de la localidad de Champotón, Campeche, México, durante el ciclo anual 2016-2017. Polibotánica, 48, 151-168. https://doi.org/10.18387/polibotanica.48.12
Gulcin, İ., Alwasel, S.H. 2023. DPPH radical scavenging assay. Processes, 11(8), 2248.
Hernández-Bernal, A.F., Gregorio-Jorge, J., León, P. 2022. El papel de los azúcares como moléculas de señalización en las plantas. TIP. Revista especializada en ciencias químico- biológicas, 25. https://doi.org/10.22201/fesz.23958723e.2022.519
Huynh, M.T., Anson, C.W., Cavell, A.C., Stahl, S.S., Hammes- Schiffer, S. 2016. Quinone 1 e-and 2 e-/2 H+ Reduction Potentials: Identification and Analysis of Deviations from Systematic Scaling Relationships. Journal of the American Chemical Society, 138(49), 15903–15910. https://doi.org/10.1021/jacs.6b05797
Jeyasanta, I., Sathish, M.N., Patterson, J. 2018. Antimicrobial susceptibility patterns of Aeromonas hydrophila from seafood sources from fish market in Tuticorin, South East coast of India. European Journal of Biomedical, 5(02), 365- 373.
Kim, D.H., Mahomoodally, M.F., Sadeer, N.B., Seok, P.G., Zengin, G., Palaniveloo, K., Khalil, A.A., Rauf, A., Rengasamy, K.R. 2020. Nutritional and bioactive potential of seagrasses: A review. South African Journal Of Botany, 137, 216-227. https://doi.org/10.1016/j.sajb.2020.10.018
Mantiniotou, M., Athanasiadis, V., Kalompatsios, D., Bozinou, E., Lalas, S.I. 2025. Therapeutic Capabilities of Triterpenes and Triterpenoids in Immune and Inflammatory Processes: A Review. Compounds, 5(1), 2. https://doi.org/10.3390/compounds5010002
Martins, G.R., Monteiro, A.F., do Amaral, F.R.L., da Silva, A.S.A. 2021. A validated Folin-Ciocalteu method for total phenolics quantification of condensed tannin-rich açaí (Euterpe oleracea Mart.) seeds extract. Journal of Food Science and Technology, 58(12), 4693-4702.
Mendoza-Isaza, N.A., Arbeláez, J.A.H., Pelaez-Jaramillo, C.A. 2020. Capacidad antioxidante y contenido de polifenoles totales de extractos de tallo de Stevia rebaudiana en varios modelos in vitro. https://www.redalyc.org/journal/1492/149264860006/html/
Núñez-Moreira, R., Quintana-Ricardo, L., Gutiérrez-Cuesta, R., Valdés-Iglesias, O., González-García, K.L., Hernandez- Rivera, Y., Acosta-Suarez, Y., Ortiz-Guillarte, E. 2019. Optimización del proceso de extracción de compuestos fenólicos de la angiosperma marina Thalassia testudinum. Revista Colombiana de Biotecnología, 21(2), 109-117.
Pérez, M., Dominguez-López, I., Lamuela-Raventós, R.M. 2023. The chemistry behind the folin–ciocalteu method for the estimation of (poly) phenol content in food: Total phenolic intake in a mediterranean dietary pattern. Journal of agricultural and food chemistry, 71(46), 17543-17553.
Putra, I. M.W.A., Suarjana, N. 2025. Evaluation of phosphomolybdenum and ferric reducing antioxidant power assays in extract combinations of Mangifera indica and Euphorbia hirta. Walisongo Journal of Chemistry, 8(1), 57-66.
Quispe, R.D., Argani, O. 2014. Fundamentos de bromatología. Rev. Act. Clin. Med. vol.41, pp. 2128-2132. 2304-3768.
Rahman, M.M., Rahaman, M.S., Islam, M.R., Rahman, F., Mithi, F.M., Alqahtani, T., Uddin, M.S. 2021. Role of phenolic compounds in human disease: current knowledge and future prospects. Molecules, 27(1), 233.
Ricco, R.A., Agudelo, I.J., Wagner, M.L. 2015. Métodos empleados en el análisis de los polifenoles en un laboratorio de baja complejidad. Lilloa, 161-174.
Shen, N., Wang, T., Gan, Q., Liu, S., Wang, L., Jin, B. 2022. Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food Chemistry, 383, 132531. https://doi.org/10.1016/j.foodchem.2022.132531
Thapa, S., Bhandari, A., Ghimire, R., Xue, Q., Kidwaro, F., Ghatrehsamani, S., Maharjan, B., Goodwin, M. 2021b. Managing Micronutrients for Improving Soil Fertility, Health, and Soybean Yield. Sustainability, 13(21), 11766. https://doi.org/10.3390/su132111766
Wołosiak, R., Drużyńska, B., Derewiaka, D., Piecyk, M., Majewska, E., Ciecierska, M., Pakosz, P. 2021. Verification of the conditions for determination of antioxidant activity by ABTS and DPPH assays—A practical approach. Molecules, 27(1), 50.
Yero-Espinosa, L., Rodríguez-Fernández, Y., Pérez-Sosa, N.D. 2017. Caracterización fitoquímica y bromatológica de la Thalassia testudinum usada en alimentación de ovinos pelibuey de Cuba (Ovis aries). Ciencia & Futuro, 7(3), 25-36.