Production of polyhydroxybutyrate from milk whey fermentation by Bacillus megaterium TRQ8
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Resumen
Los plásticos que son producidos a partir del petróleo representan un problema ambiental importante, debido a su largo periodo de degradación, altas emisiones de CO2 y por el consumo de recursos no renovables. Por lo tanto, es necesario generar alternativas sostenibles para producir polímeros naturales, como el polihidroxibutirato (PHB), que es un biopolímero producido a partir de la fermentación de residuos agroindustriales llevada a cabo por algunos microorganismos.
El objetivo de este trabajo fue analizar la composición óptima del medio de cultivo y las condiciones de cultivo para mejorar el rendimiento de la síntesis de PHB en Bacillus megaterium TRQ8. La fermentación se realizó dentro de un biorreactor con condiciones controladas y se utilizó suero de leche como fuente de carbono. Inicialmente se hizo un pretratamiento con la finalidad de eliminar los nutrientes del suero y posteriormente se llevó a cabo la fermentación y extracción del PHB; además, diferentes medios de cultivo que contenían etanol y acetato de sodio a distintas concentraciones fueron analizados con el objetivo de incrementar la producción de polihidroxibutirato. Con base en los resultados obtenidos se concluyó que el medio con etanol al 1% v / v fue el que aumentó significativamente la producción de PHB.
Detalles del artículo
Cómo citar
Ruiz Aguirre, J. B. (2017). Production of polyhydroxybutyrate from milk whey fermentation by Bacillus megaterium TRQ8. Revista Latinoamericana De Recursos Naturales, 13(1), 24-31. Recuperado a partir de https://revista.itson.edu.mx/index.php/rlrn/article/view/261
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Artículos Originales
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Andrady, A. and Neal, M. A., 2009. Applications and societal benefits of plastics. Philosophical Transactions of the Royal Society B: Biological Sciences, 364(1526): 1977-1984.
Babruwad, P., Prabhu, S., Upadhyaya, K. and Hungund, B., 2015. Production and characterization of thermostable polyhydroxybutyrate from Bacillus cereus PW3A. Biochem Tech, 3: 990-995.
Baron, S. (1996). Medical Microbiology. Galveston, USA: Elvesier.
Bell, D., Hoare, M. and Dunnill, P., 1983. The Formation of Protein Precipitates and Their Centrifugal Recovery. Torrington Place, London: University College London, T.
Bovo, F., Tuanny, L., Eliana, R. and Fernandes, C., 2014. Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1. 19/06/17, de Department of Food Engineering, College of Animal Science and Food Engineering, University of São Paulo Sitio web: http://www.scielo.br/scielo.php?script=sci_arttextypid=S0101-20612014000300019
Campuzano, A. and Vasquez, H., 2013. Evaluation of the PHB Production from Milk Whey. 28/1/17, de Universidad Nacional de Colombia Web Site: http://uest.ntua.gr/tinos2015/proceedings/pdfs/campuzano_higuita.pdf
Cyras, V., Soledad, M., Mauri, A. and Vázquez, A., 2007. Biodegradable Double-Layer Films Based on Biological Resources: Polyhydroxybutyrate and Cellulose. Wiley InterScience, 106: 749-756.
de los Santos Villalobos, S.; Parra Cota, F.I.; Herrera Sepúlveda, A.; Valenzuela Aragón, B.; Estrada Mora, J.C. 2017. COLMENA: Colección de Microorganismos Edáficos y Endófitos Nativos, para contribuir a la seguridad alimentaria nacional. Revista Mexicana de Ciencias Agrícolas. En prensa.
Fei, T., Cazeneuve, S., Wen, Z., Wu, L. and Wang, T., 2016. Effective Recovery of Poly-b-Hydroxybutyrate (PHB) Biopolymer from Cupriavidus necator Using a Novel and Environmentally Friendly Solvent System. Food Science and Human Nutrition, 4: 678-685.
Forbes, B., Sahm, D. and Weissfeld, A., 2009. Diagnóstico Microbiológico. Bueno Aires, Argentina: Médica Panamericana.
Gautam, S, 2009. Biodegradable Plastics Impact on Environment. 04/02/17, de Ministry of Environment y Forests Government of India Web Site: http://cpcb.nic.in/upload/NewItems/NewItem_150_PlasticsWaste.pdf
Getachew, A. and Woldesenbet, F., 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes, 9: 1-9.
González, Y., Meza, J., González, O. and Córdova, J., 2013. Síntesis y biodegradación de polihidroxialcanoatos: Plásticos de origen microbiano. Revista Internacional de Contamaminación Ambiental, 29(1): 77-115.
Guifang, W., Tian, B., Zhongyao, S. and Qingyu, W., 2002. Sodium Acetate Stimulates PHB Biosynthesis in Synechocystis sp. PCC 6803*. Tsinghua Science and Technology, 7: 435 - 438
Heinrich, D., Madkour, M., Al-Ghamdi, M., Shabbaj, I. and Steinbüchel, A., 2012. Large scale extraction of poly (3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite. 09/02/17, de NCBI Web Site: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564783/
Ingram, L., 1990. Ethanol tolerance in bacteria. Critical Reviews in Biotechnology, 9: 305-319.
Lambert S., 2013. Environmental Risk of Polymers and their Degradation Products. Submitted for the degree of Doctor of Philosophy. University of York Environment Department.
Lemos, A. and Mina, A., 2015. Polyhydroxyalkanoates (PHA’s) produced by bacteria its potential application to industrial level. Informador Técnico. 79(1): 93-101.
Luef, K., Stelzer, F. and Wiesbrockb, F., 2015. Poly(hydroxyalkanoates in Medical Applications. Chemistry and Technology of Materials, 29 (2): 287-297.
North, E. J. and Halden, R. U., 2013. Plastics and Environmental Health. The Road Ahead. Reviews on Environmental Health, 28(1): 1-8.
Martínez, G. and Loperena, L., 2013. Producción de plásticos biodegradables en bacterias. Thesis for Biotechnology Engineering. Facultad de ciencias Universidad de la República.
Myint, B., Champakalakshmi, R., Kishore, M., Sing, C. and Sakharkar, K., 2012. Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species. Association of Microbiologists of India, 52(3): 411-419.
Mikkili, I., Karlapudi, A., Venkateswarulu, T., Babu, J., Nath, S. and Kodali, V., 2014. Isolation, Screening and Extraction of Polyhydroxybutyrate (PHB) producing bacteria from Sewage sample. International Journal of PharmTech Research, 6: 850-857
Naranjo, J., 2010. Producción de polihidroxibutirato a partir de residuos agroindustriales. Thesis for Magister in Chemical Engineering (20 - 40). Manizales, Colombia.
Nath, A., Dixit, M., Bandiya, A., Chavda, S. and Desai, A., 2008. Enhanced PHB production and scale up studies using cheese whey in fed batch culture of Methylobacterium sp. ZP24. Bioresource Technology, 99: 5749-5755.
Obruca, S., Marova, I., Svoboda, Z. and Mikulikova, R., 2010. Use of Controlled Exogenous Stress for Improvement of Poly(3-hydroxybutyrate) Production in Cupriavidus necator. Folia Microbiology, 55 (1): 17-22.
Obruca, S., Marova, I., Stankova, M., Mravcova, L. and Svoboda, Z., 2010. Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16. World Journal of Microbiology and Biotechnology, 26:1261-1267.
Obruca, S., Marova, I., Melusova, S. and Mravcova, L., 2011. Production of polyhydroxyalkanoates from cheese whey employing Bacillus megaterium CCM 2037. Springer-Verlag, 61:947-953
Oeding, V. and Schlegel, H., 1973. β-Ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-β-hydroxybutyrate metabolism. Biochemical Journal, 134(1): 239-248.
Peña, C., Castillo, T., García, A., Millán, M. and Segura, D., 2014. Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microbial Biotechnology, 7(4): 278-293.
Rivera, L. and Nevárez, G., 2009. Economic carbon sources for bacterial bioplastic production. Tecnociencia Chihuahua. Vol., III (2): 58-63.
Seoane, I., Manfredi, L. and Cyras, V., 2013. Properties and processing relationship of polyhydroxybutyrate and cellulose biocomposites. Procedia Materials Science, 8: 808-813.
Serrano, J., 2010. Polyhydroxyalkanoates (PHAs) polymers produced by microorganisms. A solution to environmental pollution. Teoría y praxis investigativa, 5 (2): 79-84.
Singh, R., 2015. International Journal of Pharmaceutical Research | Apr-Jun 2015 | Vol 7 | Issue 2 | 17 Review Article Polyhydroxybutyrate (PHB): Biodegradable, Bioplastics Produced by Microorganisms. International Journal of Pharmaceutical Research, 7: 17-23.
Stahlhut, R. W., Welshons, W. V., and Swan, S. H., 2009. Bisphenol A Data in NHANES Suggest Longer than Expected Half-Life, Substantial Nonfood Exposure, or Both. Environmental Health Perspectives, 117(5): 784-789.
Talsness, C. E., Andrade, A. J. M., Kuriyama, S. N., Taylor, J. A., and vom Saal, F. S., 2009. Components of plastic: experimental studies in animals and relevance for human health. Philosophical Transactions of the Royal Society B: Biological Sciences, 364(1526): 2079-2096.
Thompson, R. C., Swan, S. H., Moore, C. J., and vom Saal, F. S, 2009. Our plastic age. Philosophical Transactions of the Royal Society B: Biological Sciences, 364 (1526): 1973-1976.
Thompson, R. C., Moore, C. J., vom Saal, F. S., and Swan, S. H, 2009. Plastics, the environment and human health: current consensus and future trends. Philosophical Transactions of the Royal Society B: Biological Sciences, 364 (1526): 2153-2166.
Tokiwa, Y., Calabia, B. P., Ugwu, C. U. and Aiba, S., 2009. Biodegradability of Plastics. International Journal of Molecular Sciences, 10(9): 3722-3742.
Tóth, M. E., Vígh, L. and Sántha, M, 2014. Alcohol stress, membranes, and chaperones. Cell Stress y Chaperones, 19(3): 299-309.
Valencia, E. and Ramírez, M., 2009. La industria de la leche y la contaminación del agua. Elementos. (73), (27-31). Puebla, México.
Vary, PS., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W. and Jahn, D., 2007. Bacillus megaterium from simple soil bacterium to industrial protein production host. Applied Microbiology and Biotechnology, 76: 957-967.
Wang, L., Zhu, W., Wang, X., Chen, X., Chen, G. and Xu, K., 2007. Processability Modifications of Poly (3-hydroxybutyrate) by Plasticizing, Blending, and Stabilizing. Journal of Applied Polymer Science, 107: 166-173.
Webb, H., Arnott, J., Crawford, R. and Ivanova, E., 2013. Plastic Degradation and Its Environmental Implications with Special Reference to Poly(ethylene terephthalate). Polymers, 5: 1-18.
Babruwad, P., Prabhu, S., Upadhyaya, K. and Hungund, B., 2015. Production and characterization of thermostable polyhydroxybutyrate from Bacillus cereus PW3A. Biochem Tech, 3: 990-995.
Baron, S. (1996). Medical Microbiology. Galveston, USA: Elvesier.
Bell, D., Hoare, M. and Dunnill, P., 1983. The Formation of Protein Precipitates and Their Centrifugal Recovery. Torrington Place, London: University College London, T.
Bovo, F., Tuanny, L., Eliana, R. and Fernandes, C., 2014. Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1. 19/06/17, de Department of Food Engineering, College of Animal Science and Food Engineering, University of São Paulo Sitio web: http://www.scielo.br/scielo.php?script=sci_arttextypid=S0101-20612014000300019
Campuzano, A. and Vasquez, H., 2013. Evaluation of the PHB Production from Milk Whey. 28/1/17, de Universidad Nacional de Colombia Web Site: http://uest.ntua.gr/tinos2015/proceedings/pdfs/campuzano_higuita.pdf
Cyras, V., Soledad, M., Mauri, A. and Vázquez, A., 2007. Biodegradable Double-Layer Films Based on Biological Resources: Polyhydroxybutyrate and Cellulose. Wiley InterScience, 106: 749-756.
de los Santos Villalobos, S.; Parra Cota, F.I.; Herrera Sepúlveda, A.; Valenzuela Aragón, B.; Estrada Mora, J.C. 2017. COLMENA: Colección de Microorganismos Edáficos y Endófitos Nativos, para contribuir a la seguridad alimentaria nacional. Revista Mexicana de Ciencias Agrícolas. En prensa.
Fei, T., Cazeneuve, S., Wen, Z., Wu, L. and Wang, T., 2016. Effective Recovery of Poly-b-Hydroxybutyrate (PHB) Biopolymer from Cupriavidus necator Using a Novel and Environmentally Friendly Solvent System. Food Science and Human Nutrition, 4: 678-685.
Forbes, B., Sahm, D. and Weissfeld, A., 2009. Diagnóstico Microbiológico. Bueno Aires, Argentina: Médica Panamericana.
Gautam, S, 2009. Biodegradable Plastics Impact on Environment. 04/02/17, de Ministry of Environment y Forests Government of India Web Site: http://cpcb.nic.in/upload/NewItems/NewItem_150_PlasticsWaste.pdf
Getachew, A. and Woldesenbet, F., 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes, 9: 1-9.
González, Y., Meza, J., González, O. and Córdova, J., 2013. Síntesis y biodegradación de polihidroxialcanoatos: Plásticos de origen microbiano. Revista Internacional de Contamaminación Ambiental, 29(1): 77-115.
Guifang, W., Tian, B., Zhongyao, S. and Qingyu, W., 2002. Sodium Acetate Stimulates PHB Biosynthesis in Synechocystis sp. PCC 6803*. Tsinghua Science and Technology, 7: 435 - 438
Heinrich, D., Madkour, M., Al-Ghamdi, M., Shabbaj, I. and Steinbüchel, A., 2012. Large scale extraction of poly (3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite. 09/02/17, de NCBI Web Site: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564783/
Ingram, L., 1990. Ethanol tolerance in bacteria. Critical Reviews in Biotechnology, 9: 305-319.
Lambert S., 2013. Environmental Risk of Polymers and their Degradation Products. Submitted for the degree of Doctor of Philosophy. University of York Environment Department.
Lemos, A. and Mina, A., 2015. Polyhydroxyalkanoates (PHA’s) produced by bacteria its potential application to industrial level. Informador Técnico. 79(1): 93-101.
Luef, K., Stelzer, F. and Wiesbrockb, F., 2015. Poly(hydroxyalkanoates in Medical Applications. Chemistry and Technology of Materials, 29 (2): 287-297.
North, E. J. and Halden, R. U., 2013. Plastics and Environmental Health. The Road Ahead. Reviews on Environmental Health, 28(1): 1-8.
Martínez, G. and Loperena, L., 2013. Producción de plásticos biodegradables en bacterias. Thesis for Biotechnology Engineering. Facultad de ciencias Universidad de la República.
Myint, B., Champakalakshmi, R., Kishore, M., Sing, C. and Sakharkar, K., 2012. Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species. Association of Microbiologists of India, 52(3): 411-419.
Mikkili, I., Karlapudi, A., Venkateswarulu, T., Babu, J., Nath, S. and Kodali, V., 2014. Isolation, Screening and Extraction of Polyhydroxybutyrate (PHB) producing bacteria from Sewage sample. International Journal of PharmTech Research, 6: 850-857
Naranjo, J., 2010. Producción de polihidroxibutirato a partir de residuos agroindustriales. Thesis for Magister in Chemical Engineering (20 - 40). Manizales, Colombia.
Nath, A., Dixit, M., Bandiya, A., Chavda, S. and Desai, A., 2008. Enhanced PHB production and scale up studies using cheese whey in fed batch culture of Methylobacterium sp. ZP24. Bioresource Technology, 99: 5749-5755.
Obruca, S., Marova, I., Svoboda, Z. and Mikulikova, R., 2010. Use of Controlled Exogenous Stress for Improvement of Poly(3-hydroxybutyrate) Production in Cupriavidus necator. Folia Microbiology, 55 (1): 17-22.
Obruca, S., Marova, I., Stankova, M., Mravcova, L. and Svoboda, Z., 2010. Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16. World Journal of Microbiology and Biotechnology, 26:1261-1267.
Obruca, S., Marova, I., Melusova, S. and Mravcova, L., 2011. Production of polyhydroxyalkanoates from cheese whey employing Bacillus megaterium CCM 2037. Springer-Verlag, 61:947-953
Oeding, V. and Schlegel, H., 1973. β-Ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-β-hydroxybutyrate metabolism. Biochemical Journal, 134(1): 239-248.
Peña, C., Castillo, T., García, A., Millán, M. and Segura, D., 2014. Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microbial Biotechnology, 7(4): 278-293.
Rivera, L. and Nevárez, G., 2009. Economic carbon sources for bacterial bioplastic production. Tecnociencia Chihuahua. Vol., III (2): 58-63.
Seoane, I., Manfredi, L. and Cyras, V., 2013. Properties and processing relationship of polyhydroxybutyrate and cellulose biocomposites. Procedia Materials Science, 8: 808-813.
Serrano, J., 2010. Polyhydroxyalkanoates (PHAs) polymers produced by microorganisms. A solution to environmental pollution. Teoría y praxis investigativa, 5 (2): 79-84.
Singh, R., 2015. International Journal of Pharmaceutical Research | Apr-Jun 2015 | Vol 7 | Issue 2 | 17 Review Article Polyhydroxybutyrate (PHB): Biodegradable, Bioplastics Produced by Microorganisms. International Journal of Pharmaceutical Research, 7: 17-23.
Stahlhut, R. W., Welshons, W. V., and Swan, S. H., 2009. Bisphenol A Data in NHANES Suggest Longer than Expected Half-Life, Substantial Nonfood Exposure, or Both. Environmental Health Perspectives, 117(5): 784-789.
Talsness, C. E., Andrade, A. J. M., Kuriyama, S. N., Taylor, J. A., and vom Saal, F. S., 2009. Components of plastic: experimental studies in animals and relevance for human health. Philosophical Transactions of the Royal Society B: Biological Sciences, 364(1526): 2079-2096.
Thompson, R. C., Swan, S. H., Moore, C. J., and vom Saal, F. S, 2009. Our plastic age. Philosophical Transactions of the Royal Society B: Biological Sciences, 364 (1526): 1973-1976.
Thompson, R. C., Moore, C. J., vom Saal, F. S., and Swan, S. H, 2009. Plastics, the environment and human health: current consensus and future trends. Philosophical Transactions of the Royal Society B: Biological Sciences, 364 (1526): 2153-2166.
Tokiwa, Y., Calabia, B. P., Ugwu, C. U. and Aiba, S., 2009. Biodegradability of Plastics. International Journal of Molecular Sciences, 10(9): 3722-3742.
Tóth, M. E., Vígh, L. and Sántha, M, 2014. Alcohol stress, membranes, and chaperones. Cell Stress y Chaperones, 19(3): 299-309.
Valencia, E. and Ramírez, M., 2009. La industria de la leche y la contaminación del agua. Elementos. (73), (27-31). Puebla, México.
Vary, PS., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W. and Jahn, D., 2007. Bacillus megaterium from simple soil bacterium to industrial protein production host. Applied Microbiology and Biotechnology, 76: 957-967.
Wang, L., Zhu, W., Wang, X., Chen, X., Chen, G. and Xu, K., 2007. Processability Modifications of Poly (3-hydroxybutyrate) by Plasticizing, Blending, and Stabilizing. Journal of Applied Polymer Science, 107: 166-173.
Webb, H., Arnott, J., Crawford, R. and Ivanova, E., 2013. Plastic Degradation and Its Environmental Implications with Special Reference to Poly(ethylene terephthalate). Polymers, 5: 1-18.